Acta Veterinaria Hungarica 44 (1) (1996)
Bovine Leukocyte Adhesion Deficiency: A brief overview of a modern disease and its implications.
Gerardi, A. S.
Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary.
Nagy, B., Nagy, Gy., Meder, Magdolna and Mocsári, E.
Mycoplasmas associated with bovine conjunctivitis and keratoconjunctivitis.
Naglić, T., Sanković, F., Madić, J., Hajsig, D., eol, Branka and Busch, Kristina
Data on the contamination of maize with fumonisin B1 and other fusariotoxins in Hungary.
Fazekas, B., Kis, M. and Tóth Hajdu, Edit
Factors influencing microbial growth and the efficiency of microbial protein synthesis: A review.
Fébel, Hedvig, and Fekete, S.
Complex study of the physiological role of cadmium IV. Effects of prolonged dietary exposure of broiler chickens to cadmium.
Bokori, J., Fekete, S., Glávits, R., Kádár, I. and Kővári, L.
Haematologic values of Hyla rabbits fed different levels of substrate used for the propagation of Pleurotus pulmonarius.
Rupić, V., Boac, R., Muic, S., Romić, . and Liker, B.
The effect of Iloprost on peroxidation in dog ischaemic myocardium.
Ogonowska-Kobusiewicz, Maria, Kobusiewicz, W., Żywicki, W., Michalak, J., lepko, J. and Ledwożyw, A.
Lack of acid-resistant trypsin inhibitor in mares colostrum: Short communication.
Baintner, K. and Csapó, J.
Effect of vitamins A and E on blood plasma vitamin status and daily body mass gain of different fat-tailed sheep breeds.
Asadian, A., Mézes, M., and Mirhadi, S. A.
Therapeutic efficacy of providone-codine (Betadine) and dichloroxylenol (Septocid) in Holstein cows affected with endometritis and/or cervicitis.
Koujan, A., Eissa, H. M., Hussein, M. A., Ayoub, M. M. and Afiefy, M. M.
Comparative clinical and biochemical analyses of two methods of pancreatic cannulation in pigs.
Brzeski, W., Depta, A., Winnicki, T. and Rychlik, A.
Acta Veterinaria Hungarica 44 (1), pp. 1-8 (1996)
BOVINE LEUKOCYTE ADHESION DEFICIENCY: A BRIEF OVERVIEW OF A MODERN DISEASE AND ITS IMPLICATIONS
A. S. GERARDI
94 Sheilas Way, Lynn, MA 01904, U.S.A.
(Received September 15, 1995; accepted November 10, 1995)
Bovine Leukocyte Adhesion Deficiency (BLAD) is a genetic disease of cattle affecting the hematopoietic system. In the last decade BLAD has become a disease of economic importance in the dairy industry. As such, this overview describes the chronological developments and thinking that led to the elucidation of BLAD as a distinct disease entity from previous models in canine and human populations. All species affected exhibit symptoms of chronic and recurrent infections. Necrotic and/or gangrenous infections of soft tissues are prevalent, as well as secondary infections with bacteria or fungi. Low birthweight and unthriftiness are key symptoms of neonates in all species affected by LAD. Dermatomycoses and impaired pus formation are also common findings. The physiological basis for BLAD is a deficiency in leukocyte (particularly neutrophil) chemotactic and phagocytic properties. The inhibition of diapedesis in the inflammatory response prevents normal immune reactions to invading pathogens. Chronic infections are a consequence of the faulty immune mechanisms. The biochemical etiology of BLAD involves cell surface glycoprotein molecules known as integrins. These are responsible for cell cell interactions necessary for neutrophils to adhere to vascular endothelium in a normal individual. Experiments using monoclonal antibodies to block LFA-1, Mac-1, and p150,95 (three integrins vital for cell cell interactions) mimic BLAD symptomatology and have led to the discovery of the reciprocal Intercellular Adhesion Molecule (ICAM). Through pedigree analysis and biochemical detection with restrictive endonucleases BLAD has been isolated genetically to a single gene locus. The economic significance and prophylaxis are briefly discussed. In addition, the beneficial aspects of the study of BLAD are addressed. There are advantages of producing a BLAD-like state in preventing transplant rejection, ischemia-reperfusion injury, and other scenarios arising from the deleterious effects of the inflammatory response.
Key words: Bovine Leukocyte Adhesion Deficiency (BLAD), hematopoietic system, leukocyte, neutrophil, integrins, secondary infections
Acta Veterinaria Hungarica 44 (1), pp. 9-19 (1996)
ENTEROTOXIGENIC ESCHERICHIA COLI, ROTAVIRUS, PORCINE EPIDEMIC DIARRHOEA VIRUS, ADENOVIRUS AND CALICI-LIKE VIRUS IN PORCINE POSTWEANING DIARRHOEA IN HUNGARY
B. NAGY1, GY. NAGY2, Magdolna MEDER2 and E. MOCSÁRI2
1Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H 1581 Budapest, P.O. Box 18, Hungary;
2Central Veterinary Institute, H 1149 Budapest, Tábornok u. 2, Hungary
(Received January 4, 1996; accepted January 29, 1996)
In order to obtain data about the significance of enterotoxigenic E. coli (ETEC), and of the different enteric viruses in the aetiology of porcine postweaning diarrhoea, bacteriological, electron microscopic and ELISA studies were made on dead and live pigs. E. coli from the small intestine of diarrhoeal weaned pigs that died were tested for serogroups, pili (fimbriae) and toxin-genotype. The 108 haemolytic E. coli representing 14 farms and 42 pigs were typed as follows: 0149:K88+ETEC (56.5%), OX:K88+ETEC (21.3%), O141:F18ac+ETEC (4.6%), O147:F18ac+ETEC (3.7%), OX and O157:F18ac+ETEC (9.3%), verotoxigenic O141:F18ac (2%). In another study, when faecal samples of 92 live diarrhoeal weaned pigs (representing 19 farms) were tested, rotaviruses (18.6%), porcine epidemic diarrhoea virus (PED) (5.5%) and calici-like viruses (5.5%), and adenovirus (two pigs) were detected, besides K88+ETEC (12%) and K99+ or 987P+ETEC (one of each). Combined infections were detected in 9% of the samples. Sequential studies of diarrhoeal and nondiarrhoeal weaned and unweaned pigs indicated that PED virus and group A rotavirus were related to diarrhoea but adeno- or calici-like virus were not. It was concluded that K88+ETEC was the overwhelming aetiologic agent of porcine postweaning diarrhoea in Hungary, but F18ac+ETEC, group A rotavirus and PED virus were also significant.
Key words: Enterotoxigenic E. coli, enteric viruses, rotavirus, PED-coronavirus, adenovirus, calicivirus, pigs
Acta Veterinaria Hungarica 44 (1), pp. 21-24 (1996)
MYCOPLASMAS ASSOCIATED WITH BOVINE CONJUNCTIVITIS AND KERATOCONJUNCTIVITIS
T. NAGLIĆ1, F. SANKOVIĆ2, J. MADIĆ1, D. HAJSIG1, Branka EOL1 and Kristina BUSCH3
1Department of Microbiology and Infectious Diseases, Veterinary Faculty, University of Zagreb, 10000 Zagreb, Heinzelova 55, Croatia; 2Clinic of Surgery, Veterinary Faculty, University of Zagreb, 10000 Zagreb, Heinzelova 55, Croatia;
3PLIVA d.d., Zagreb, Research Institute, 10000 Zagreb, Prilaz baruna Filipović a 25, Croatia
(Received July 20, 1995; accepted October 27, 1995)
In two separate herds of fattening calves a sudden-onset outbreak of ocular disease with profuse lacrimation occurred. The disease resembled the early stage of infectious bovine keratoconjunctivitis but after a few days the clinical signs of bronchopneumonia appeared. From conjunctival swabs Mycoplasma (M.) bovigenitalium, M. bovirhinis and infectious bovine rhinotracheitis (IBR) virus were isolated. Moraxella bovis infection was not established. In one of the herds M. bovigenitalium was also found in the pneumonic lungs of dead calves. In one herd M. bovoculi was isolated from a cow with chronic keratoconjunctivitis, housed together with affected calves. Mycoplasmas were not isolated from ocular swabs of six bulls originating from a Reproductive Centre with temporary occurrence of unilateral serous conjunctivitis resistant to antibiotic therapy.
Key words: Bovine eyes, Mycoplasma bovigenitalium, M. bovirhinis, M. bovoculi, IBR virus
Acta Veterinaria Hungarica 44 (1), pp. 25-37 (1996)
DATA ON THE CONTAMINATION OF MAIZE WITH FUMONISIN B1 AND OTHER FUSARIOTOXINS IN HUNGARY
B. FAZEKAS1, M. KIS2 and Edit TÓTH HAJDU1
1Veterinary Institute of Debrecen, H4031 Debrecen, Bornemissza u. 37, Hungary;
2Debrecen University Medical School, H4012 Debrecen, Nagyerdei krt. 98, Hungary
(Received October 18, 1995; accepted November 14, 1995)
The fumonisin B1, zearalenone, deoxynivalenol and T-2 toxin content of maize samples collected in the period of storage and harvesting was determined by high-performance liquid chromatography (HPLC) methods. Of the mouldy maize samples collected in the period of storage, 70.8% contained fumonisin B1(0.0519.8 mg/kg; average concentration: 2.6 mg/kg), 87.5% contained zearalenone (0.0111.8 mg/kg, average concentration: 1.26 mg/kg), 70% contained deoxynivalenol (0.0721.2, average concentration: 4.33 mg/kg), and 41.7% contained T-2 toxin (0.060.39, average concentration: 0.2 mg/kg). These mycotoxins often occurred together in the samples. In the non-mouldy samples, both the positivity rate and the mycotoxin concentration were markedly lower. In the harvesting period, the mycotoxin content of maize ears more or less affected by moulds (so-called affected sample) and of the average sample was determined separately for each maize-field involved in the study. Of the affected samples, 70% contained fumonisin B1 (0.09552.4 mg/kg; average: 6.64 mg/kg), 17% contained zearalenone (0.0060.079 mg/kg; average: 0.03 mg/kg), 13% contained deoxynivalenol (0.050.118 mg/kg; average: 0.09 mg/kg), and 39% contained T-2 toxin (0.050.551 mg/kg; average: 0.165 mg/kg). Fumonisin B1 and T-2 toxin often occurred together in the affected maize samples. Of the average samples, 30% were contaminated with fumonisin B1 (0.065.1 mg/kg; average: 1.52 mg/kg) and 9% with T-2 toxin (min.-max.-average: 0.05 mg/kg). The results call attention to the fact that maize cultivated in Hungary is often contaminated with fumonisin B1. High concentrations of fumonisin B1 were found primarily in the mouldy or affected maize ears; therefore, the development of diseases caused by fumonisin B1 should be reckoned with primarily if such maize is fed. Besides fumonisin B1, mouldy or affected maize usually contains also other fusariotoxins, which raises the possibility of mycotoxin interactions. Because of the high prevalence of fumonisin B1 in maize grown in Hungary, the authors suggest that samples of maize used for feeding horses and pigs should be checked for fumonisin B1 content.
Key words: Mycotoxin, fusariotoxin, fumonisin B1, maize
Acta Veterinaria Hungarica 44 (1), pp. 39-56 (1996)
FACTORS INFLUENCING MICROBIAL GROWTH AND THE EFFICIENCY OF MICROBIAL PROTEIN SYNTHESIS: A REVIEW
Hedvig FÉBEL1 and S. FEKETE2
1Research Institute for Animal Breeding and Nutrition, H2053 Herceghalom, Hungary;
2Department of Animal Nutrition, University of Veterinary Science, H1400 Budapest, P. O. Box 2, Hungary
(Received April 6, 1995; accepted July 21, 1995)
The possibilities of influencing microbial growth and the efficiency of microbial protein synthesis are reviewed. The authors present a detailed discussion of the effects exerted by the level of feed intake, the feeding frequency, the concentrate to forage ratio, the quality of the carbohydrate and protein source, and the interaction of carbohydrate and protein on microbial protein synthesis and, consequently, on the flow of microbial protein. An in-depth knowledge of the above factors influencing bacterial growth and microbial fermentation is essential for the well-balanced feeding of ruminants (primarily dairy cows) of high production level.
Key words: Microbial growth and efficiency, carbohydrates, proteins, feed, rumen, degradability
Acta Veterinaria Hungarica 44 (1), pp. 57-74 (1996)
COMPLEX STUDY OF THE PHYSIOLOGICAL ROLE OF CADMIUM IV. EFFECTS OF PROLONGED DIETARY EXPOSURE OF BROILER CHICKENS TO CADMIUM
J. BOKORI1, S. FEKETE1, R. GLÁVITS2, I. KÁDÁR3, J. KONCZ3 and L. KŐVÁRI1
1Department of Animal Nutrition, University of Veterinary Science, H1400 Budapest, P.O. Box 2, Hungary; 2Central Veterinary Institute, Budapest, Hungary; 3Agrochemical and Plant Nutrition Department, Soil Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary
(Received June 21, 1995; accepted September 29, 1995)
A 274-day long cadmium (Cd) feeding trial was carried out with broiler cockerel chickens. The diet of the control group (Cd-0, n = 6) contained no added Cd, whereas to the diet of group Cd-25 and group Cd-75 (n = 10 each) 25 ppm and 75 ppm Cd was added, respectively, in the form of CdSO4. The chickens were subjected to examinations described in detail earlier (Bokori et al., 1995b). In addition, the relative mass of five different organs (heart, liver, testis, spleen and brain), expressed as % of the body mass, was also determined. The clinical, gross and histopathological examinations and the assay of organs for mineral element content led to the following main findings. The feeding of diets containing 25 or 75 ppm Cd for more than 9 months did not cause signs indicative of acute Cd toxicosis or mortality in either group. The body mass gain of group Cd-75 chickens markedly decreased. Prolonged Cd exposure of the cockerels increased the relative mass of the liver and heart and markedly decreased that of the testes. The change in mass was proportional to the Cd load. The Cd-fed chickens developed focal pathological fatty infiltration of the liver, histiocytic infiltration of the jejunal mucosa and focal lympho-histiocytic interstitial infiltration and fibrosis of the kidney, which supports the view that prolonged Cd exposure leads to the development of subacute-chronic tissue changes in the kidney. The Cd content of the organs increased by one to three orders of magnitude, in direct proportion to the Cd load. The Cd content of most organs was 2 to 3 times as high as the value reported for broilers exposed to a similar Cd load lasting for 68 days (Bokori et al., 1995b). This indicates that the degree of Cd accumulation is markedly influenced by the duration of the Cd exposure. The highest Cd content was demonstrated in the kidney (724 mg/kg of dry matter). The Cd exposure markedly lowered the Zn, Mo and B content of the bones and the Ni content of the myocardium.
Key words: Broiler chickens, cadmium load, clinical signs, cadmium accumulation, gross lesions, histopathological changes
Acta Veterinaria Hungarica 44 (1), pp. 75-83 (1996)
HAEMATOLOGIC VALUES OF HYLA RABBITS FED
DIFFERENT LEVELS OF BY-PRODUCT ARISING FROM THE PROPAGATION OF PLEUROTUS PULMONARIUS
V. Rupić1, R. Boac1, S. Muic1, . Romić2 and B. Liker1
1Faculty of Agriculture, University of Zagreb; Svetoimunska c. 25, 41000 Zagreb, Croatia;
2New Hospital, Aleja Izviđač a, Zagreb, Croatia
(Received August 14, 1995; accepted November 12, 1995)
The nutritive influence of different levels of by-product arising from the propagation of the mushroom Pleurotus pulmonarius on the red and white blood cell count of rabbits was studied in the course of fattening. The experiment was carried out on 4 groups of male Hyla rabbits, a control group (C) and 3 experimental groups (E1, E2, E3), with 15 rabbits per group. The control group was fed a standard fodder and the three experimental groups were fed the standard mix with 10%, 20% or 30% dried by-product added, respectively. At the end of the 9th week of the experiment, blood samples were obtained by cardiac puncture, and the red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrit, thrombocyte count, white blood cell (WBC) count and the differential WBC were determined. The different levels of by-product interwoven by mycelium added to the feed did not change the RBC count but significantly increased the MCV, MCH, haemoglobin concentration and the haematocrit value. The different diets had no influence on either the WBC count or the differential WBC count, but the number of thrombocytes decreased significantly.
Key words: Hyla rabbits, feeding, by-product of mushroom growing, Pleurotus pulmonarius, haematologic parameters, red blood cell count, white blood cell count
Acta Veterinaria Hungarica 44 (1), pp. 85-93 (1996)
THE EFFECT OF ILOPROST ON PEROXIDATION IN DOG ISCHAEMIC MYOCARDIUM
Maria OGONOWSKA-KOBUSIEWICZ2, W. KOBUSIEWICZ1*, W. ŻYWICKI1, J. MICHALAK1, J. LEPKO1 and A. LEDWOŻYW3
1Clinic of Vascular Surgery, 2Department of Anaesthesiology, Medical Academy, Jaczewskiego 8, 20 950 Lublin, Poland;
3Department of Pathophysiology, Agricultural Academy, Lublin, Poland
(Received April 20, 1995; accepted September 25, 1995)
The aim of this study was to investigate the effect of Iloprost (Schering AG), a stable prostacyclin analogue, on the intensity of thiobarbituric acid-reactive substances (TBARS), conjugated diene and hydroperoxide production in ischaemic dog myocardium. Adult male mongrel dogs were divided into groups as follows: A = sham/operated group, B = control animals, and C = Iloprost-supplemented group. The drug was administered as a single intravenous bolus at a dose of 10 ľg/kg. Partial heart ischaemia was induced by occlusion of the left descending coronary artery. After 3 h ischaemia the dogs were killed, their hearts were removed and the levels of the above-mentioned peroxidation products were assayed. The concentrations of TBARS, conjugated dienes and hydroperoxides were significantly increased not only in the ischaemic region, but also in the border zone and in the normally perfused region of the heart. A considerable decrease of these products was observed in the Iloprost-treated group. A direct effect of Iloprost on the heart cell membranes is postulated.
Key words: Heart, ischaemia, peroxidation, Iloprost
*to whom correspondence should be addressed
Acta Veterinaria Hungarica 44 (1), pp. 95-97 (1996)
LACK OF ACID-RESISTANT TRYPSIN INHIBITOR IN MARES COLOSTRUM: SHORT COMMUNICATION
K. BAINTNER and J. CSAPÓ
Department of Physiology and Chemistry, Faculty of Animal Science,
Pannon Agricultural University, H 7400 Kaposvár, P.O. Box 16, Hungary
(Received December 1, 1995; accepted December 28, 1995)
Mare s colostrum was collected and examined for the presence of trypsin inhibitors. It was found to contain a low level of trypsin inhibitor which could be denatured by 2.5% trichloroacetic acid and, therefore, it clearly differs from the acid-resistant colostral inhibitor of Artiodactyla and Carnivora. This finding is exceptional for a species that concentrates IgG in the colostrum and whose newborn absorbs colostral proteins non-selectively by the gut. It appears that the presence of colostral trypsin inhibitor is not essential for the transmission of maternal immunity via the colostrum and the gut.
Key words: Horse, colostrum, trypsin inhibitor, immune transmission, IgG, newborn, Artiodactyla, Perissodactyla, Carnivora, Ungulata
Acta Veterinaria Hungarica 44 (1), pp. 99-109 (1996)
EFFECT OF VITAMINS A AND E ON BLOOD PLASMA VITAMIN STATUS AND DAILY BODY MASS GAIN OF DIFFERENT FAT-TAILED SHEEP BREEDS
A. ASADIAN1,2, M. MÉZES1* and S. A. MIRHADI2
1Department of Nutrition, University of Agricultural Sciences, H 2103 Gödöllő,
Páter K. u. 1, Hungary; 2Animal Husbandry Research Institute, 31585 Karaj, Iran
(Received July 5, 1995; accepted October 13, 1995)
Ninety ram lambs of three Iranian fat-tailed sheep breeds including the Shal breed (age: 4 6 months) in one experiment and the Sanjabi and Bakhtiari breeds (6 9 months) in another experiment were assigned to five groups of 5 7 lambs each to receive a basal diet plus different levels of vitamins A and E supplementation. The groups received 0, 50 or 100 IU vitamin A/kg body mass (b.m.) and 0, 15 or 30 IU vitamin E/kg of diet. In the vitamin A groups a constant level of 15 IU vitamin E and in the vitamin E groups a constant level of 50 IU vitamin A supplementation was provided. Blood plasma samples were taken at the beginning and at the end of the experiments and the concentrations of vitamins A and E were determined by high-performance liquid chromato-graphy (HPLC). Vitamin A supplementation of the highest level (100 IU/kg b.m.) significantly increased the retinol content of the blood plasma in the Shal (P < 0.01) and Bakhtiari (P < 0.05) breeds. The mild increase of retinol concentration in the Sanjabi breed was not significant (P > 0.05). Vitamin A supplementation greatly decreased the a -tocopherol content of the blood plasma in all three breeds (P < 0.01). It increased the average daily body mass gain of lambs of the Shal breed (P < 0.01) but decreased that of Bakhtiari lambs (P < 0.05). Supplementation of vitamin E mildly decreased the retinol content of the blood plasma in all the breeds and the decrease was statistically significant (P < 0.05) for the Shal breed at the highest dose level. Vitamin E supplementation greatly increased the a -tocopherol concentration of the blood plasma in all the breeds (P < 0.01 0.001). It decreased the average daily body mass gain of the Shal breed (P < 0.001) but improved it in the two other breeds. That increase was statistically significant (P < 0.05) in the Bakhtiari breed. It was concluded that the Shal breed gave a better response to vitamin A supplementation while the Sanjabi and Bakhtiari breeds to vitamin E. This indicates that fat-tailed breeds have dissimilar requirements for vitamins A and E supplementation. A higher level of vitamin E intake is required for raising the a -tocopherol content of the blood plasma above the level critical for sheep (0.8 mg/ml).
Key words: Fat-tailed sheep, retinol, tocopherol, body mass gain
*to whom correspondence should be addressed
Acta Veterinaria Hungarica 44 (1), pp. 111-119 (1996)
THERAPEUTIC EFFICACY OF POVIDONE-IODINE (BETADINE) AND DICHLOROXYLENOL SEPTOCID) IN HOLSTEIN COWS AFFECTED WITH ENDOMETRITIS AND/OR CERVICITIS
A. KOUJAN2, H. M. EISSA1* , M. A. HUSSEIN3, M. M. AYOUB1 and M. M. AFIEFY1
1Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; 2Department of Surgery and Obstetrics, Faculty of Veterinary Medicine, Baath University, Hama, Syria; 3General Organization of Veterinary Services, Dokki, Giza, Egypt
(Received November 28, 1994; accepted October 30, 1995)
The efficacy of either povidone-iodine (Betadine) or dichloroxylenol (Septocid) intrauterine infusions on the treatment of endometritis and/or cervicitis in cows was examined. One hundred and twelve repeat-breeder Holstein cows (aged 37 years) were selected for this study. Rectal and vaginal examinations were applied to detect the signs of endometritis and/or cervicitis. Cows were assigned into two groups: the first group (n = 60) was treated with Betadine solution (0.5%) while the second group (n = 52) was given Septocid (0.1%) administered as intrauterine infusion (100150 ml). Both treatments were repeated at least two times at 7-day intervals and the cows were rechecked. The responding animals were inseminated 1012 h after oestrus detection using frozen semen from bulls of proven fertility. The success or failure of treatment was evaluated on the basis of the post-treatment conception rate. The relationship of the body weight of cows, the length of the service period (open days) and the number of previous services with the results of treatment with Betadine or Septocid is discussed. The recovery and conception rates obtained after Betadine treatment were better than those obtained after Septocid. Moreover, healthy cows (3 500 kg body weight) and those inseminated before post-partum day 180, having no more than 47 previous services, responded well to either Betadine or Septocid treatment.
Key words: Endometritis, cervicitis, Holstein cows, povidone-iodine (Betadine), dichloroxylenol (Septocid), intrauterine infusion
*to whom correspondence should be addressed
Acta Veterinaria Hungarica 44 (1), pp. 121-129 (1996)
COMPARATIVE CLINICAL AND BIOCHEMICAL ANALYSES OF TWO METHODS OF PANCREATIC CANNULATION IN PIGS
W. BRZESKI1, A. DEPTA2, T. WINNICKI1 and A. RYCHLIK2
1Department of Surgery and Radiology and 2Division of Clinical Diagnostic
Department of Internal Diseases, Academy of Agriculture and Technology,
10-719 Olsztyn, Poland
(Received April 12, 1995; accepted October 30, 1995)
The authors method of long-lasting pancreatic cannulation from the side of the duodenal papilla was compared with the method of accessory pancreatic duct direct cannulation in 20 pigs divided into two groups of 10 animals each. The volume of pancreatic juice was determined in both groups. In addition, the parameters of acid-base equilibrium in whole blood and the values of Na+, K+, Cl, pH and HCO3 in the pancreatic juice were determined. More efficient drainage was obtained with the authors method. However, due to the longer collection of pancreatic juice multielectrolytic compensation liquids had to be used in the animals to maintain acid-base equilibrium and to prevent the risk of metabolic acidosis.
Key words: Pancreas, cannulation, pigs, pancreatic juice