Acta Veterinaria Hungarica 44 (1) (1996)
Histopathological changes induced by environmental stress in common carp, Japanese coloured carp, European eel, and African catfish.
Cytogenetic effects of the herbicide chloridazon in cultured sheep lymphocytes.
Katarína iviková and J. Dianovský
Characterisation of the swine swC1 antigen.
A. Magyar and R. Mihalik
Infectious disease control
Serological findings obtained in cattle herds immunised with the Brucella melitensis Rev.1 and the B. abortus B19 vaccine in Mongolia.
Role of the Danish pig production system in the success of infectious disease control: A review.
Olive by-products in pig fattening.
V. Rupić, I. Jerković, R. Boac, Dunja Glowattzky, S. Muic and V. Hrabak
Comparison of indirect immunofluorescence test (IIF) and enzyme-linked immunosorbent assay (ELISA) in detecting Babesia bigemina infection in cattle.
G. G. B. Reddy, A. K. Mishra, J. R. Rao and A. K. Tewari
Some haematological and immunological parameters of farmed deer in Hungary.
Z. Zomborszky, Éva Horn, S. Tuboly and P. Gyódi
Brain-mediated protective interactions of histaminergic H2 and dopaminergic systems in rats.
Grabarević, I. Rotkvić, P. Sikirić, S. Seiwerth, P. Daja, J. Perić and J. Kos
The effect of b-carotene and vitamins A, D3 and E on some reproductive parameters in cows.
Stefania Iwańska and Danuta Strusińska
Acta Veterinaria Hungarica 45 (1), pp. 1-10 (1997)
HISTOPATHOLOGICAL CHANGES INDUCED BY ENVIRONMENTAL STRESS IN COMMON CARP, JAPANESE COLOURED CARP, EUROPEAN EEL, AND AFRICAN CATFISH
Department of Pathology and Forensic Veterinary Medicine,
University of Veterinary Science, H1400 Budapest, P.O. Box 2, Hungary
(Received January 14, 1997; accepted February 6, 1997)
Histopathological changes caused by stress during catching and transport were examined in four fish species, the common carp (Cyprinus carpio), the Japanese coloured carp (koi, the coloured variant of the Asian carp, Cyprinus carpio haematopterus), the European eel (Anguilla anguilla), and the African catfish (Clarias gariepinus). In all species, the goblet cells of the gills and skin were decreased in number and slight detachment of the epithelium of the secondary gill lamellae was seen. Loss of goblet cells and detachment of the columnar epithelial cells were found in the intestinal mucosa of common carp and coloured carp. It is supposed that these lesions may affect ion transport and respiration in the gills and disturb normal intestinal function, thus serving as a starting point for different diseases.
Key words: Environmental stress effect, common carp, Japanese coloured carp, European eel, African catfish, histopathological changes, loss of mucus, detachment of epithelial cells
Acta Veterinaria Hungarica 45 (1), pp. 11-16 (1997)
CYTOGENETIC EFFECTS OF THE HERBICIDE CHLORIDAZON IN CULTURED SHEEP LYMPHOCYTES
Katarína IVIKOVÁ and J. DIANOVSKÝ
Department of Veterinary Genetics, University of Veterinary Medicine, Komenského 73, 04181 Koice, Slovak Republic
(Received July 15, 1996; accepted January 22, 1997)
The effect of in vitro exposure to the herbicide chloridazon on the induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) was studied in cultured sheep peripheral lymphocytes. A positive clastogenic effect was observed in chloridazon-treated cultures at a dose of 7 × 104 M both in the presence and absence of the S9 fraction, but no significant increase of chromosome breaks was seen at lower doses (7 × 106 M and 7 × 105 M, respectively). A clear dose-dependence and significant differences were found in chloridazon potency to induce SCEs. Induction of cell cycle delays as compared to the controls was not observed.
Key words: Chloridazon, sheep peripheral lymphocytes, chromosome aberrations, sister chromatid exchanges, S9 fraction
Acta Veterinaria Hungarica 45 (1), pp. 17-31 (1997)
CHARACTERISATION OF THE SWINE swC1 ANTIGEN
A. MAGYAR1* and R. MIHALIK2
1Department of Biochemistry, Laboratory of Embryology, Loránd Eötvös University,
H2131 Göd, Jávorka u. 14, Hungary; 2Department of Immunology, National Institute of Haematology,
H1113 Budapest, Daróczi u. 24, Hungary
(Received October 14, 1996; accepted February 6, 1997)
Some biochemical and functional characteristics of the swine swC1 antigen, determined by the use of the authors swC1-specific monoclonal antibody (mAb) 335-2, are reported. The molecular weight of the antigen was determined by immunoprecipitation. The swC1 antigen has 41 and approx. 15 kD components under reducing conditions. It is sensitive to proteolytic enzymes such as bromelain or trypsin, but not to papain. Phosphatidylinositol-specific phospholipase C treatment diminished the expression of swC1 on the surface of leukocytes. Cross-linking of swC1 on the cell surface did not influence the proliferation of mitogen-activated mononuclear cells and had no mitogenic activity by itself. During 48 h of mitogen activation its surface expression did not change significantly. Possible relationships of swC1 to human CD antigens are discussed in the light of the results obtained.
Key words: Swine leukocytes, cell surface antigens, swC1, immunoprecipitation, PI-anchor
Acta Veterinaria Hungarica 45 (1), pp. 33-43 (1997)
SEROLOGICAL FINDINGS OBTAINED IN CATTLE HERDS IMMUNISED WITH THE BRUCELLA MELITENSIS REV.1 AND THE B. ABORTUS B19 VACCINE IN MONGOLIA
Central Veterinary Institute, H1581 Budapest, P.O. Box 2, Hungary
(Received September 16, 1996; accepted January 27, 1997)
To reduce the economic losses caused by Brucella abortus infection under the conditions of nomadic cattle breeding, more than half million cattle over 3 months of age (excluding male animals) were immunised with the B. melitensis Rev.1 vaccine in 7 provinces of Mongolia in the first year of a programme (1987). In the second year, only heifer calves over 3 months of age, yearling animals not vaccinated in the first year, as well as heifers over 2 years of age and cows were vaccinated. The vaccine was administered subcutaneously at a dose of 5 × 109 viable organisms. In co-operatives that had used the B. abortus B19 vaccine, the existing vaccination programme was maintained, and all cattle over 3 months of age (except male animals) were vaccinated with a dose of 5 × 1010 viable organisms, using the same vaccination programme as in herds immunised with the Rev.1 vaccine. Before vaccination, two serological tests were carried out at an interval of 2025 days to determine the incidence of Brucella infection. Serum samples were tested by the Rose Bengal test (RBT), serum agglutination test (SAT) and complement fixation test (CFT). An infection rate of 3.835% was found in the herds tested. Animals serologically positive for brucellosis were removed from the herds. Fifteen to 21 days after vaccination, blood samples were taken from 10% of the animals in all immunised herds. The serum samples were tested by SAT and CFT to check the seroconversion rate. In herds immunised with the Rev.1 vaccine 90% of the cows and 92.7% of the heifers were seropositive, while the seropositivity rate of B19-vaccinated cows and heifers was 68.8% and 89.7%, respectively. The serological status of the vaccinated animals was checked not only immediately after vaccination but also 12 months thereafter (in B19-vaccinated cows also 24 months after vaccination). After Rev.1 vaccination, antibodies were present in 7% of the cows, 4.6% of the 3-year-old heifers, 3.1% of the 2-year-old heifers, and 1.6% of the 1-year-old animals. By comparison, 13.2% of the cows vaccinated with the B. abortus B19 vaccine were serologically positive 2 years after immunisation. As regards the B19-vaccinated heifers, 7.9%, 5% and 2.7% of the 3-year-old, 2-year-old and 1-year-old animals, respectively, were serologically positive even 12 months after vaccination.
Key words: Brucella melitensis Rev.1, Brucella abortus B19, vaccine, brucellosis, cattle, serology, Mongolia
Acta Veterinaria Hungarica 45 (1), pp. 45-51 (1997)
ROLE OF THE DANISH PIG PRODUCTION SYSTEM IN THE SUCCESS OF INFECTIOUS DISEASE CONTROL: A REVIEW
Department of Microbiology and Infectious Diseases, University of Veterinary Science, H1143 Budapest, Hungária krt. 23, Hungary
(Received November 27, 1996; accepted February 10, 1997)
Pigs produced by the Danish system have played a dominant role on the international market for many years. A key factor in attaining that position was that the producers recognised the importance of animal health in market regulation and profit generation in due time. This paper reviews the factors that, through a close co-operation between the production and animal health sectors, have contributed to the stable market position of Danish pigs.
Key words: Pig, farm management, disease control, economics, Denmark
Acta Veterinaria Hungarica 45 (1), pp. 53-66 (1997)
OLIVE BY-PRODUCTS IN PIG FATTENING
V. RUPIĆ1, I. JERKOVIĆ2, R. BOAC1, Dunja GLOWATTZKY2, S. MUIC1 and V. HRABAK2
1Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Svetoimunska 25, Croatia; 2PIK Vrbovec, Gradec Pig Farm, Croatia
(Received October 25, 1996; accepted February 10, 1997)
The utilisation in pig fattening of diets with various proportions of dried olive cake, i.e., olive by-product resulting from centrifugal separation, was investigated in 60 Swedish Landrace × Large White crossbreds (30 castrates and 30 gilts). The pigs were divided into three equal groups (10 + 10): two experimental groups, fed with a fodder mix containing 50 g/kg and 80 g/kg respectively of dried olive cake, and a control group, fed with the same mix but minus the cake. The experiment lasted 90 days. For the first 45 days the pigs were given the starter, and for the second 45 days the finisher, mix. Throughout the whole period, pigs in both experimental groups achieved greater average body mass and mass gain than the control animals. Concurrently, castrates in all three groups became heavier and demonstrated greater mass gain than did the gilts. While supplied respectively with the finisher mix throughout the whole test period, pigs fed mixes with 50 g/kg of dried olive cake demonstrated significantly greater feed consumption than those fed without the cake and than those fed mixes with 80 g/kg of cake. While supplied with the starter mix, pigs fed mixes with 50 g/kg of dried olive cake achieved the lowest feed conversion rate, whereas those supplied with the finisher mix achieved the highest. Throughout the entire period no significant differences were observed in feed conversion rate among pig groups.
Key words: Pig, feeding, fattening performance, olive by-products
Acta Veterinaria Hungarica 45 (1), pp. 67-74 (1997)
COMPARISON OF INDIRECT IMMUNOFLUORESCENCE (IIF) AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) IN DETECTING BABESIA BIGEMINA INFECTION IN CATTLE
G. G. B. REDDY, A. K. MISHRA, J. R. RAO and A. K. TEWARI
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India
(Received June 26, 1996; accepted January 13, 1997)
An indirect immunofluorescence test (IIF) and an enzyme-linked immunosorbent assay (ELISA) were standardised to investigate the prevalence of bovine babesiosis caused by Babesia bigemina in experimentally and naturally infected bovids. Both IIF and ELISA detected antibodies to B. bigemina 7 days after experimental infection with 87.5% and 100% sensitivity, respectively. The IIF results indicated that a titre greater than 1:64 was a reliable indicator of B. bigemina infection. Serological study of 214 serum samples collected from Boophilus microplus infested cattle from the State of Orissa revealed 33.6% overall seroreactivity by ELISA, whereas IIF recorded 9.4%. Both IIF and ELISA showed some degree of cross-reactivity between Indian (Izatnagar) and Mexican strains of B. bigemina.
Key words: Babesia bigemina, IIF, ELISA
Acta Veterinaria Hungarica 45 (1), pp. 75-84 (1997)
SOME HAEMATOLOGICAL AND IMMUNOLOGICAL PARAMETERS OF FARMED DEER IN HUNGARY
Z. ZOMBORSZKY1, Éva HORN2, S. TUBOLY3 and P. GYÓDI1
1Faculty of Animal Science, Pannon University of Agriculture, H7401 Kaposvár, P.O. Box 16, Hungary; 2Veterinary Institute of Kaposvár; 3Department of Microbiology and Infectious Diseases, University of Veterinary Science, Budapest, Hungary
(Received January 24, 1997; accepted February 27, 1997)
A haematological survey of farmed fallow deer (Dama dama) and red deer (Cervus elaphus) was carried out. In the winter period, the red blood cell (RBC) count was 9.04 × 1012/L in adult fallow-bucks, 8.83 × 1012/L in fallow-does, and 6.07 × 1012/L in fawns. The haemoglobin (Hb) level and mean corpuscular volume (MCV) were also significantly (P < 0.05) lower in fawns than in adult fallow deer. Using blood samples taken from red deer in different physiological and nutritional periods, in stags the lowest mean RBC count was measured in January, at the time of casting the antlers (4.68 × 1012/L), while in the hinds in the suckling period (6.27 × 1012/L). The mean RBC count of samples taken from a group of red deer fawns at six different times between 1 week and 1 year of age varied between 5.80 and 7.14 × 1012/L. While the RBC count showed minor variations, the Hb concentration was almost constant in the different periods in both species. The coefficient of correlation between RBC and Hb was 0.66 and 0.58 in fallow deer and red deer, respectively. Studying the blastogenic transformation of lymphocytes by immunological tests, a higher rate of response to nonspecific mitogens (Phaseolus vulgaris, PHA; concanavalin A, Con A) was found in farmed deer (> 30%) than in deer captured in the wild and kept under farm conditions for 10 or 13 months (< 20%).
Key words: Haematology, fallow deer, red deer, blastogenic transformation
Acta Veterinaria Hungarica 45 (1), pp. 85-93 (1997)
BRAIN-MEDIATED PROTECTIVE INTERACTIONS OF HISTAMINERGIC H2 AND DOPAMINERGIC SYSTEMS IN RATS
GRABAREVIĆ1, I. ROTKVIĆ2, P. SIKIRIĆ3, S. SEIWERTH4, P. DAJA5, J. PERIĆ5
and J. KOS6
1Department of General Pathology and Pathological Morphology, Veterinary Faculty, University of Zagreb, 41000 Zagreb, Heinzelova 55, Croatia; 2Division of Gastroenterology, University Hospital Sestre Milosrdnice , Zagreb; 3Department
of Pharmacology, School of Medicine, Zagreb; 4Department of Pathology, School
of Medicine, Zagreb; 5Department of Forensic Veterinary Medicine and Department
of Surgery, Veterinary Faculty, University of Zagreb, Croatia
(Received July 15, 1996; accepted December 3, 1996)
Pretreatment with dopamine agonists (bromocriptine 2.5, L-dopa 2.5, apomorphine 0.05 mg/kg i.p.) and a histamine H2 receptor antagonist (cimetidine 50.0 mg/kg i.p.) was found to greatly reduce the haemorrhagic gastric lesions induced by 15-min pylorus ligation in rats. On the other hand, pretreatment with dopamine antagonists (haloperidol 5.0, sulpiride 1.0, domperidone 5.0 mg/kg i.p.) significantly aggravated these lesions. Cimetidine markedly diminished the ulcerogenic effect of haloperidol but not that of domperidone, suggesting a brain-mediated site for the protective interaction of cimetidine and dopamine systems.
Key words: Interaction, protective effect, dopaminergic system, cimetidine, dopamine agonists and antagonists, haemorrhagic gastric lesions
Acta Veterinaria Hungarica 45 (1), pp. 95-107 (1997)
THE EFFECT OF b -CAROTENE AND VITAMINS A, D3 AND E ON SOME REPRODUCTIVE PARAMETERS IN COWS
Stefania IWAŃSKA and Danuta STRUSIŃSKA
Institute of Animal Nutrition and Food Economics, Academy of Agriculture
and Technology, 10-718 Olsztyn-Kortowo, Oczapowskiego 2, Poland
(Received July 31, 1996; accepted December 9, 1996)
Five groups of winter-housed cows (n = 10 per group) that calved in the winter were used to assess the effect of b -carotene supplementation on postpartum reproductive performance. Near parturition and immediately after calving the b -carotene concentrations of the blood plasma were decreased and no differences could be found between the control and the supplemented groups. The results obtained at postpartum day 60 suggest that supplementation of the daily winter ration with 300 mg of synthetic
b -carotene with or without vitamins A, D3 and E exerts the most favourable effect on reproduction, as judged not only from b -carotene and vitamin A contents of the blood plasma, colostrum and milk but also from the improved fertility indices. The number of inseminations per cow was reduced and the conception rate was significantly higher in cows supplied additionally with 300 mg of synthetic
b -carotene with or without vitamins A, D3 and E. It can be concluded that b -carotene is an important factor in bovine reproduction and that its specific role cannot be taken over by vitamin A.
Key words: Cow, reproduction, b -carotene, vitamin A, blood plasma, colostrum, milk