VOLUME 46, NUMBER 2, 1998

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Acta Veterinaria Hungarica 46 (2) (1998)

CONTENTS AND ABSTRACTS

Animal breeding

In vivo assessment of breast muscle, abdominal fat and total fat volume in meat-type chickens by magnetic resonance imaging. 
G. Kövér, R. Romvári, P. Horn, E. Berényi, J. F. Jensen and P. Sorensen
135

Endocrinology

Thyroid volumetric measurement and quantitative thyroid scintigraphy in dogs. 
L. Balogh, Julianna Thuróczy, I. Biksi, Margit Kulcsár, Gy. A. Jánoki, P. Rudas and Gy. Huszenicza
145

Diagnosis of hyperadrenocorticism in dogs as compared to human diagnostic methods: A review. 
Julianna Thuróczy, L. Balogh, Gy. Huszenicza, Gy. A. Jánoki and Margit Kulcsár
157

Endocrinology of pregnancy: Chorionic somatomammotropins and pregnancy-associated glycoproteins: A review. 
J. F. Beckers, A. Zarrouk, E. S. Batalha, J. M. Garbayo, L. Mester and O. Szenci
175

Immunology

Comparison of 3H-thymidine incorporation and CellTiter 96™ AQueous colorimetric assays in cell proliferation of bovine mononuclear cells. 
Anna Zolnai, E. B. Tóth, R. A. Wilson and V. L. Frenyó
191

Infectious diseases

Value of different serological tests in the diagnosis of bovine brucellosis in the Amazonian region. 
Éva Molnár, L. Molnár and W. G. Vale
199

Microbiology

Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1a isolated from ducks and pigs. 
S. Bernáth, Gy. Morovján, V. Sztojkov and G. Szita
211

Bacteriological investigations on wound myiasis of sheep caused by Wohlfahrtia magnifica (Diptera: Sarcophagidae).
Erika Tóth, R. Farkas, K. Márialigeti and I. S. Mokhtar
219

Nutrition

Effects of phytase supplementation on calcium and phosphorus output, production traits and mechanical stability of the tibia in broiler chickens. 
Margit Vetési, M. Mézes, Györgyi Baskay and E. Gelencsér
231

Relationship of dietary fat and lysine level with body composition in broiler chickens. 
I. Szakáll, S. Fekete, Emese Andrásofszky, R. Romvári and G.  Szita
243

Parasitology

Studies on the occurrence of actinosporean stages of fish myxosporeans in a fish farm of Hungary, with the description of triactinomyxon, raabeia, aurantiactinomyxon and neoactinomyxon types.
Amina El-Mansy, Cs. Székely and K. Molnár
259

Physiology

Effects of different hybrids, strains and age of laying hens on the cholesterol content of the table egg. 
G. Kovács, K. Dublecz, F. Husvéth, L. Wágner, D. Gerendai, J. Orbán and H. Manilla 
285

Surgery

Clinical renal allograft transplantation in a Dalmatian dog: Case report.
T. Németh, J. Tóth, L. Balogh, Gy. Jánoki, F. Manczur, K. Vörös and G. Dallos
295


Acta Veterinaria Hungarica 46 (2), pp. 135-–144 (1998)

IN VIVO ASSESSMENT OF BREAST MUSCLE, ABDOMINAL  FAT AND TOTAL FAT VOLUME IN MEAT-TYPE CHICKENS BY MAGNETIC RESONANCE IMAGING

G. Kövér1, R. Romvári1*, P. Horn1, E. Berényi1, J. F. Jensen2 and P. Sorensen3

1Faculty of Animal Science, Pannon Agricultural University, P.O. Box 16, 
H-7400 Kaposvár, Hungary; 2Institute of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Bülowsvej 13, 1870 Fredericksberg C, Denmark; 3Danish Institute of Animal Science, P.O. Box 39, 8830 Tjele, Denmark

(Received November 18, 1997; accepted January 6, 1998)

Measurements were performed on commercial broiler chickens by magnetic resonance imaging (MRI) tomography. A total of 72 chickens were scanned at the age of 6, 7, 8, 10, 16 and 20 weeks. MRI scans based on spin echo sequence were taken in the three orthogonal planes. The volume of the pectoral muscles increased from 259 cm3 to 1035 cm3 in males and from 250 cm3 to 875 cm3 in females between 6 and 20 weeks of age. Abdominal fat volume increased from 18.9 cm3 to 153.2 cm3 in males and from 19.3 cmto 267.0 cm3 in females between 7 and 20 weeks of age. Total body fat volume increased from 129 cm3 to 557 cm3 in males and from 171 cm3 to 1321 cm3 in females between 6 and 20 weeks of age. The correlation coefficients between the total volume of fat pixels and the amount of abdominal fat determined in the coronal plane by MRI were 0.85 and 0.95 in the male and female sex, respectively. Changes found in the pectoral muscles (mm. pectorales) as well as in total and abdominal fat volume were in close agreement with dissection data obtained at the slaughterhouse and with laboratory data based on the measurement of total body chemical composition in both sexes. This MRI method can be a good complement to computed tomography (CT) scanning in the transversal plane.

Key words: Chicken, magnetic resonance imaging (MRI), pectoral muscle, abdominal fat, total body fat

*Corresponding author


Acta Veterinaria Hungarica 46 (2), pp. 145–-156 (1998)

THYROID VOLUMETRIC MEASUREMENT AND QUANTITATIVE THYROID SCINTIGRAPHY IN DOGS

L. Balogh1, Julianna Thuróczy2, I. Biksi3, Margit Kulcsár2, Gy. A. Jánoki1
P. Rudas4 and Gy. Huszenicza2

1Department of Applied Radioisotopes, National Frederic Joliot-Curie Research 
Institute for Radiobiology and Radiohygiene, H-1775 Budapest, P.O. Box 101, Hungary; 2Department of Obstetrics and Gynaecology, 3Department of Pathology, and
4Department of Physiology and Biochemistry, University of Veterinary Science, 
H-1400 Budapest, P.O. Box 2, Hungary

(Received September 19, 1997; accepted January 6, 1998)

Thyroid volumetric measurement combined with quantitative 99mTc-pertechnetate thyroid scintigraphy was performed in 62 clinical canine patients having suspected thyroid abnormalities. Euthyroid dogs (n = 22) had a total thyroid size of 3.60 ą 1.36 cm3, the thyroid/salivary gland region of interest (ROI) ratio was 2.01 ą 0.55, the thyroid/background ROI ratio was 3.86 ą 0.90, and 20-min thyroid radioactivity uptake was 1.17 ą 0.71% of the injected dose (I. D.). By Student’s unpaired test, thyroid size of the hypothyroid group (n = 36) was not statistically different from that of the euthyroid dogs, but all other quantitative data (e.g. thyroid/salivary gland ROI ratio = 1.08 ą 0.56, thyroid/background ROI ratio = 2.32 ą 0.70, and 20-min thyroid radioactivity uptake = 0.34 ą 0.22% of the I. D.) were significantly (p < 0.001) lower in hypothyroid than in euthyroid dogs. Evaluating the above-listed quantitative data of separated thyroid lobes by Student’s paired test, there was no significant difference between the left and the right lobe either in the euthyroid or in the hypothyroid group.

Key words: 99mTc-pertechnetate quantitative thyroid scintigraphy, thyroid size evaluation, volumetric measurements, TRH stimulation test, skin biopsy, euthyroid, hypothyroid, hyperthyroid, thyroid malignancies, dog


Acta Veterinaria Hungarica 46 (2), pp. 157–-173 (1998)

DIAGNOSIS OF HYPERADRENOCORTICISM IN DOGS AS COMPARED TO HUMAN DIAGNOSTIC METHODS: A REVIEW

Julianna Thuróczy1, L. Balogh2, Gy. Huszenicza1, Gy. A. Jánoki2 and Margit Kulcsár1

1Department of Obstetrics and Reproduction, University of Veterinary Science,
H-1400 Budapest, P.O. Box 2, Hungary; 2Department of Applied Radioisotopes, 
National Frédéric Joliot-Curie Research Institute for Radiobiology and Radiohygiene, H-1775 Budapest, P.O. Box 101, Hungary

(Received October 3, 1997; accepted January 28, 1998)

Spontaneous hyperadrenocorticism is a common and well-recognised endocrine disorder occurring in dogs. The biochemical diagnosis of hyperadrenocorticism rests on the documentation of excessive glucocorticoid levels or metabolites in urine or blood. The diagnosis of canine hyperadrenocorticism is historically developed from human methods and based on non-dynamic and dynamic tests and visualisation. In some cases the different ranges of endocrine parameters or metabolites necessitate the modification of human protocols. Elevation of the activity of alkaline phosphatase (AP) and its heat-resistant isoenzyme (SIAP) induced by endogenous or exogenous glucocorticoid excess raise the suspicion of hyperadrenocorticism. Cortisol values in morning urine are related to creatinine concentrations to correct for differences in urine concentration. Theoretically, the administration of dexamethasone (DX) at a relatively low dose (0.01 mg/kg of body weight) can inhibit the pituitary secretion of ACTH and, in turn, decrease endogenous cortisol secretion for as long as 24 to 48 h. Therefore, DX administration to dogs with a functioning adrenocortical tumour would not affect the plasma cortisol concentration at any time following its administration. The high-dose dexamethasone suppression test (HDDS) is based on the observation that the function of adrenocortical tumours is independent of pituitary ACTH and they completely suppress ACTH secretion; therefore, regardless of its dose, dexamethasone is never able to suppress cortisol secretion. HDDS can be combined with the measurement of urinary cortisol/creatinine (c/c) ratio from morning urine samples on three consecutive days. In case of non-suppressible urinary c/c ratio one has to speculate on differentiating adrenal tumour (AT) from non-suppressible pituitary-dependent hyperadrenocorticism (PDH) due to a pituitary tumour arising from the intermediate lobe. Radiocholesterol scintigraphy is a less frequently used technique in the diagnosis of canine Cushing’s syndrome (CCS); however, it has the same advantages in the localisation and characterisation of adrenocortical diseases as in humans.

Key words: Hyperadrenocorticism, diagnosis, humans, dogs, alkaline phosphatase (ALP), steroid-induced ALP (SIAP), urine cortisol/creatinine (c/c) ratio, adrenocorticotropic hormone (ACTH), dexamethasone suppression, scintigraphy


Acta Veterinaria Hungarica 46 (2), pp. 175–-189 (1998)

ENDOCRINOLOGY OF PREGNANCY: CHORIONIC SOMATOMAMMOTROPINS 
AND PREGNANCY-ASSOCIATED GLYCOPROTEINS: REVIEW

J. F. Beckers1, A. Zarrouk1, E. S. Batalha1, J. M. Garbayo1, L. Mester2 
and O. Szenci2*

1University of Liege, Faculty of Veterinary Medicine, Physiology of Reproduction, 
Bd de Colonster 20 B41, B-4000 Sart-Tilman, Belgium; 2University of Veterinary 
Science, Department of Obstetrics and Reproduction, Budapest, P.O. Box 2, 
H-1400 Budapest, Hungary

(Received December 5, 1997; accepted January 28, 1998)

The two main groups of placental proteins of ruminants are discussed in this paper: chorionic somatomammotropins (placental lactogens) and pregnancy-specific (-associated) proteins. Placental lactogens belong to the prolactin and growth hormone family. They stimulate mammogenesis, fetal growth and maternal metabolism. Pregnancy-specific proteins and pregnancy-associated glycoproteins belong to the aspartic proteinase family like pepsin, cathepsin D and E. These two groups of proteins are secreted in the maternal circulation by the binucleate cells after their migration to and fusion with the uterine cells. Their profiles were determined by radioimmunoassay (RIA). Further investigations are in progress to relate secretory profiles with alterations of the trophoblastic function such as those occurring in embryonic mortality, abortion, and fetal distress. The endocrine function of the primate and equine placenta is also discussed.

Key words: Chorionic somatomammotropins (placental lactogens), pregnancy-specific (-associated) proteins, cow, sheep, goat

*Corresponding author


Acta Veterinaria Hungarica 46 (2), pp. 191-–197 (1998)

COMPARISON OF 3H-THYMIDINE INCORPORATION AND CELLTITER 96TM AQUEOUS COLORIMETRIC ASSAYS IN CELL PROLIFERATION OF BOVINE MONONUCLEAR CELLS

Anna Zolnai1, E. B. Tóth2, R. A. Wilson3 and V. L. Frenyó1*

1Department of Physiology and Biochemistry, University of Veterinary Science, H-1400 Budapest, P.O. Box 2, Hungary; 2Department of Humanmorphology and Developmental Biology, Semmelweis University of Medicine, H-1094 Budapest, Tűzoltó u. 58, Hungary; 3Department of Veterinary Science, Pennsylvania State University, University Park, PA 16802, USA

(Received November 17, 1997; accepted January 28, 1998)

A rapid colorimetric non-radioactive assay for the determination of bovine mitogen-induced lymphocyte proliferation in cell culture was evaluated using a novel tetrazolium compound (MTS) and an electron coupling reagent (PMS) provided in the CellTiter 96TM kit (Promega). The results of the new method were compared with those of the 3H-thymidine incorporation assay using parallels obtained from the same lymphocyte population. The concentrations used in the cell suspension of primary cultured lymphocytes resulted in a significant signal/background ratio when cells were prepared from peripheral blood, spleen or mesenteric lymph nodes. The same concentrations of thymocytes resulted in a weak signal even for the highest concentrations of mitogen. A good correlation was demonstrated between the results of the two methods. The non-radioactive method performed well in assays in bovine mononuclear cells derived from prolactin-treated or -untreated calves, showing a 50% lower responsiveness to mitogenic stimulation in prolactin-deprived animals.

Key words: Bovine, lymphocyte, proliferation, non-radioactive, method

*Corresponding author


Acta Veterinaria Hungarica 46 (2), pp. 199–-210 (1998)

VALUE OF DIFFERENT SEROLOGICAL TESTS IN THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN THE AMAZONIAN REGION

Éva MOLNÁR*, L. MOLNÁR* and W. G. VALE

University Federation of Pará (UFPA), Centro Agropecuário, LIDEA, 
66.075-900 Campus Universitário do Guamá, Belém, Pará, Brazil

(Received July 14, 1997; accepted November 10, 1997)

The high prevalence of brucellosis is one of the most serious animal health problems affecting cattle and buffalo herds of the Amazonian region. Modern diagnostic methods are not used or not even known, and thus test results are not available in that region. Therefore, in this study a total of 878 selected sera were subjected to comparative examination by five different serological tests (buffered plate agglutination test, tube agglutination test, complement fixation test, indirect ELISA, competitive ELISA). Indirect ELISA gave the highest number of positive results, except in samples derived from the Marajó island, for which the competitive ELISA proved to be the most sensitive. The sensitivity of the classical tests (agglutination, complement fixation) was markedly lower than that of the two ELISAs. After vaccination of 2-month-old heifer calves with the B19 vaccine, all tests showed a 50–60% seropositivity, which disappeared within four months.

Key words: Bovine brucellosis, serological tests, agglutination, complement fixation, indirect ELISA, competitive ELISA, Amazonian region

* On leave from the Department of Microbiology and Infectious Diseases, University of Veterinary Science, H–1581 Budapest, P.O. Box 22, Hungary


Acta Veterinaria Hungarica 46 (2), pp. 211–-217 (1998)

COMPARISON OF THE PROTEIN COMPOSITION OF ERYSIPELOTHRIX RHUSIOPATHIAE STRAINS OF SUBTYPE 1A ISOLATED FROM DUCKS AND PIGS

S. Bernáth1, Gy. Morovján1, V. Sztojkov2 and G. Szita3

1State Control Institute for Veterinary Biologicals, Drugs and Feeds, H–1107 Budapest, Szállás u. 8, Hungary; 2Central Veterinary Institute, H-1149 Budapest, Tábornok u. 2, Hungary; 3University of Veterinary Science, H-1400 Budapest, P.O. Box 2, Hungary

(Received January 14, 1998; accepted February 23, 1998)

Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. To characterise the similarity of the strains, Pearson’s correlation coefficient was calculated. The degree of similarity between duck strains originating from the same flock was 980-991, while that of strains isolated from ducks and pigs varied between 836 and 991 on a scale of 0 to 1000. The method gives highly reproducible results and can be utilised in epidemiological investigations. Its use may provide new data on E. rhusiopathiae strains, complementing the results of methods recommended for this purpose earlier and increasing the efficiency of epidemiological investigations.

Key words: Erysipelothrix rhusiopathiae, strains, duck, pig, origin, SDS-PAGE, protein analysis, Pearson’s correlation coefficient


Acta Veterinaria Hungarica 46 (2), pp. 219-–229 (1998)

BACTERIOLOGICAL INVESTIGATIONS ON WOUND MYIASIS OF SHEEP CAUSED BY WOHLFAHRTIA MAGNIFICA (DIPTERA: SARCOPHAGIDAE)

Erika Tóth1, R. Farkas2, K. Márialigeti1 and I. S. Mokhtar1

1Department of Microbiology, Loránd Eötvös University, H–1088 Budapest, 
Múzeum krt. 4/a, Hungary; 2Department of Parasitology and Zoology, 
University of Veterinary Science, H-1400 Budapest, P.O. Box 2, Hungary

(Received August 8, 1997; accepted January 28, 1998)

The aim of the present investigation was to get further information about obligate aerobic and facultatively anaerobic bacterial communities of the intact and Wohlfahrtia magnifica infested vulval region of sheep. The numbers of aerobic and facultatively anaerobic microorganisms were lower in samples taken from uninfested mucous membrane and myiatic wounds as well as in the wound fluid as compared to samples originating from the uninfested skin surface. Gram-negative bacteria belonging to the family Enterobacteriaceae were isolated only from the skin and mucous membrane of uninfested sheep. Gram-positive microorganisms dominated in all samples. The ratio of facultatively anaerobic bacteria was higher than 80% in the sample taken from a lesion containing third instar Wohlfahrtia larvae and in the wound discharge collected from a vulval wound free of maggots. It is suggested that there is a shift in the composition of the bacterial communities of vulva as staphylococci disappear from the wounds due to the presence of Wohlfahrtia larvae.

Key words: Myiasis, bacterial communities, Wohlfahrtia magnifica


Acta Veterinaria Hungarica 46 (2), pp. 231–-242 (1998)

EFFECTS OF PHYTASE SUPPLEMENTATION ON CALCIUM AND PHOSPHORUS OUTPUT, PRODUCTION TRAITS AND MECHANICAL STABILITY OF THE TIBIA IN BROILER CHICKENS

Margit Vetési1, M. Mézes1, Györgyi Baskay1 and E. Gelencsér2

1Departments of Nutrition and 2Mechanics, Gödöllő University of Agricultural Sciences, P.O Box 303, H-2103 Gödöllő, Hungary

(Received October 20, 1997; accepted December 4, 1997)

A feeding trial was performed using 4 × 60 day-old chickens (Ross 208 cockerels) raised up to 42 days of age to determine whether exogenous phytase addition increases phosphorus utilisation by broiler chickens, and to assess its effects on some production traits as well as on the ash content and mechanical stability of the tibia. The chickens’ feed consisted of maize, wheat, soybean meal, fish meal, yeast, and fat powder. The basic feed was supplemented with inorganic phosphorus in groups A and B. In groups C and D, the amount of the inorganic phosphorus supplement (DCP) was decreased by 50%, at the same calcium/
phosphorus ratio. The 50% reduction of inorganic phosphorus supplementation represents a 20% decrease of total phosphorus. To the diets of groups B and D a phytase enzyme preparation (Phytase Novo CT™ ) was added. The calculated exogenous phytase activity was 600 FYT/kg feed. The decrease of inorganic phosphorus did not cause significant differences in the daily weight gain but lowered the feed conversion rate by 10%. Calcium and phosphorus excretion decreased by 18% and 15%, and the breaking strength of the tibia was also lower. Phytase supplementation of the feed at a lower rate of inorganic phosphorus supplementation did not cause changes in the body weight gain but improved the feed conversion rate by 5.6%. Phosphorus and calcium output decreased by 21% and 11%, respectively, but chemical composition and mechanical stability of the tibia were unaltered.

Key words: Phytase, phosphorus, calcium, tibia, mechanical stability, broiler chickens


Acta Veterinaria Hungarica 46 (2), pp. 243–-257 (1998)

RELATIONSHIP OF DIETARY FAT AND LYSINE LEVEL WITH BODY COMPOSITION IN BROILER CHICKENS

I. Szakáll1, S. Fekete1, Emese Andrásofszky1, R. Romvári2 
and G. Szita3

1Department of Animal Breeding and Nutrition and 3Department of Food Hygiene, 
University of Veterinary Science, H-1400 Budapest, P.O. Box 2, Hungary; 2Faculty 
of Animal Husbandry, Pannon University of Agricultural Sciences, H-7400 Kaposvár,
P.O. Box 16, Hungary

(Received August 4, 1997; accepted January 12, 1998)

The effect of dietary fat and/or lysine supplementation on the body composition of broiler chickens was studied. Body composition was measured by computed tomography (CT) and direct chemical analysis. Cockerel chicks from a previous experiment (Fekete et al., 1992) were used in this study. Ten chickens from each treatment group were euthanised, deep frozen and subjected to CT. Subsequently the bodies were dissected and ground to obtain homogeneous samples for chemical analysis. Supplementation of the diet with 6 g/kg lysine did not change total body composition but positively influenced the final body weight. In the treatment group receiving 40 g/kg added fat and 3 g/kg feed lysine (F-LYS-I) the higher body weight ran parallel with a higher fat content. The difference between the frozen body weight and final live body weight shows that lysine supplementation increased the water-holding capacity of muscle, which was the best in groups receiving a diet of normal energy content plus high lysine supplementation.

Key words: Lysine, dietary fat, broiler chicken, body composition, meat quality, computed tomography


Acta Veterinaria Hungarica 46 (2), pp. 259–-284 (1998)

STUDIES ON THE OCCURRENCE OF ACTINOSPOREAN STAGES OF FISH MYXOSPOREANS IN A FISH FARM OF HUNGARY, WITH THE DESCRIPTION 
OF TRIACTINOMYXON, RAABEIA, AURANTIACTINOMYXON AND NEOACTINOMYXON TYPES

Amina El-Mansy*, Cs. Székely** and K. Molnár

Veterinary Medical Research Institute, Hungarian Academy of Sciences,
H-1581 Budapest, P.O. Box 18, Hungary

(Received December 4, 1997; accepted January 12, 1998)

Actinosporean infection of the oligochaete fauna living in the mud and on the vegetation of fish ponds used for rearing common carp in polyculture was studied during a one-year survey at a fish farm in Hungary, located south of Budapest. Twenty-eight actinospore types were isolated from the oligochaetes Tubifex tubifex, Branchiura sowerbyi, Limnodrilus hoffmeisteri, Nais elinguis, and Stylaria lacustris collected during the survey, which could be classified into the triactinomyxon, raabeia, aurantiactinomyxon and neoactinomyxon groups. Drawings depicting individual actinospore types are presented on plates and their characteristic dimensions have been summarised in tables. The prevalence and seasonality of actinosporean infections observed in the various oligochaete species have been illustrated graphically. Infection by actinospores showed a pronounced seasonality. In the spring, summer and autumn the prevalence of raabeia infection in Branchiura exceeded 90%, while in the winter it dropped to 42%. A similar phenomenon could be observed for aurantiactinomyxon infection, while neoactinomyxon infection reached its peak in the autumn. In Tubifex, Limnodrilus, Nais and Stylaria species the peak of actinosporean infection occurred, with minor differences, in the spring and summer. Actinosporean infection in the individual Oligochaeta species showed much higher prevalence values than had been reported in the literature, which can be explained by the novelty of the examination technique used. It cannot be decided with absolute certainty which myxosporean developmental stage the different actinospore types described during this survey represent of the species of Myxosporea parasitic in the given ponds. This would require an experimental study for which the data presented here may serve as a basis.

Key words: Actinospore, myxosporeans, Myxozoa, survey, triactinomyxon, raabeia, aurantiactinomyxon, neoactinomyxon

* On leave from the National Institute of Oceanography and Fisheries, Cairo, Egypt; Student of the doctoral program in Zootaxonomy, Synbiology and Hydrobiology at the Loránd Eötvös University of Arts and Sciences, Budapest

** Corresponding author


Acta Veterinaria Hungarica 46 (2), pp. 285-–294 (1998)

EFFECTS OF DIFFERENT HYBRIDS, STRAINS AND AGE OF LAYING HENS ON THE CHOLESTEROL CONTENT OF THE TABLE EGG

G. Kovács1, K. Dublecz1, F. Husvéth1, L. Wágner1, D. Gerendai2, J. Orbán3 
and H. Manilla1

1Pannon University, Georgikon Faculty of Agricultural Sciences, H-361 Keszthely, 
Deák F. u. 16, Hungary; 2Small Animal Research Institute, H-2100 Gödöllő, 
Isaszegi u., Hungary; 3Pannon University, Faculty of Agricultural Sciences, 
H-9200 Mosonmagyaróvár, Vár u. 13, Hungary

(Received October 1, 1997; accepted January 22, 1998)

Three experiments were carried out to investigate the effects of age, moulting and strain of hens on the weight, total cholesterol content (TCh) and cholesterol concentration (CCh) of egg yolk. Only slight differences were found in the TCh and CCh of eggs between white and brown egg layer hybrids (Shaver SC 288 vs. Shaver SC 579) of the same age. On the other hand, significant differences (p < 0.05) were found in yolk CCh and TCh of both hybrids during the production period. The change in yolk CCh was periodical: it decreased in both hybrids until 45 weeks of age, then it increased, reaching a plateau between weeks 51 and 54, and declined again at the end of production. TCh, however, increased until 51 (Shaver SC 579) or 54 (Shaver SC 288) weeks of age, which could be attributed to the steady increase in yolk weight throughout the production period. In the eggs of Tetra-SL hens yolk weight, yolk CCh and TCh were slightly higher after moulting (at 108 weeks of age) than in the first production period (at 48 weeks of age), but the differences were not significant (p > 0.05). Significant inter-strain differences (p < 0.05) were found, however, in the yolk parameters measured. The highest values were found in the eggs of New Hampshire hens, as compared to the traditional Hungarian breeds. The eggs of traditional pure-bred hens contained a substantially higher amount of cholesterol than those produced by the hybrids.

Key words: Cholesterol, egg, laying hen, strain, production level


Acta Veterinaria Hungarica 46 (2), pp. 295–-303 (1998)

CLINICAL RENAL ALLOGRAFT TRANSPLANTATION IN A DALMATIAN DOG: CASE REPORT

T. Németh1, J. Tóth1, L. Balogh3, Gy. Jánoki3, F. Manczur2, K. Vörös2 
and G. Dallos4

1Clinic of Surgery and Ophthalmology and 2Department of Internal Medicine, 
University of Veterinary Science, H-1078 Budapest, István u. 2, Hungary; 
3National  Frédéric Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary; 4Clinic of Transplantation and Surgery, Semmelweis University Medical School, Budapest, Hungary

(Received November 7, 1997; accepted February 20, 1998)

A case of successful renal allograft transplantation performed in a two-year-old female Dalmatian dog suffering from end-stage chronic renal failure is reported. A one-year-old male German shepherd with severely injured spinal cord was used as kidney donor. Simultaneous kidney allograft transplantation combined with hypothermic initial perfusion as graft conservation was done, placing the donor kidney into the right iliac fossa of the recipient. The immunosuppression protocol consisted of prednisolone and azathioprine. Regular physical, laboratory, ultrasonographic and scintigraphic examinations were used for assessing both the morphology and the function of the allograft. After a two-week period of hospitalisation the patient was discharged in a remarkably improving condition. The recipient died on postoperative day 45 of respiratory insufficiency resulting from secondary pneumonia and pulmonary oedema. Neither macroscopic nor microscopic abnormalities of the allograft were revealed by necropsy.

Key words: Renal transplantation, dog, allograft, renal failure, renal scintigraphy