VOLUME 46, NUMBER 4, 1998

actafejlec.jpg

Acta Veterinaria Hungarica 46 (4) (1998)

CONTENTS AND ABSTRACTS

Infectious diseases

Occurrence of some viruses and bacteria involved in respiratory diseases of ruminants in Hungary.
M. Rusvai and L. Fodor
405

Microbiology

Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction. 
I. Biksi, I. Kacskovics, Mira Mándoki, Judit Iván, I. Horváth-Papp, G. Makay 
and F. Vetési 
415

Nutrition

Effect of methionine and its derivatives on the weight gain and protein utilisation of growing rats. 
M. Hegedűs, S. Fekete, Emese Andrásofszky and I. Hullár 
421

Parasitology

Detection of antibodies to Neospora caninum in bovine postabortion blood samples from Hungary. 
S. Hornok, K. Näslund, I. Hajtós, J. Tanyi, L. Tekes, I. Varga, A. Uggla and C. Björkman 
431

Studies on the occurrence of actinosporean stages of myxosporeans in Lake Balaton, Hungary, with the description of triactinomyxon, raabeia and aurantiactinomyxon types. 
Amina El-Mansy, Cs. Székely and K. Molnár 
437

Occurrence of skrjabillanid nematodes in fishes of Hungary and in the intermediate host, Argulus foliaceus L. 
K. Molnár and Cs. Székely 
451

Philometra karunensis sp. n. (Nematoda: Philometridae) from Barbus sharpeyi (Pisces) in freshwaters of Southwest Iran. 
Jamileh Pazooki and K. Molnár 
465

Physiology

3,3’,5-triiodothyronine (T3) uptake and expression of thyroid hormone receptors during the adaptation to hypothyroidism of the brain of chicken. 
B. Gereben, A. Kollár, T. Bartha, N. Buys, E. Decuypere and P. Rudas 
473

Reproduction

Endoscopic methods in swine reproductive research: A review. 
J. Rátky, K.-P. Brüssow and L. Solti 
487

Comparison of different treatments for parthenogenetic activation of bovine oocytes matured in vitro
Sz. Bodó, A. Dinnyés, B. Baranyai, L. Solti and J. Dohy 
493

Progesterone determination in equine plasma using different immunoassays. 
P. Nagy, L. Solti, Margit Kulcsár, J. Reiczigel, Gy. Huszenicza, Katalin Abaváry and Anna Wölfling 
501

Book reviews 
515


Acta Veterinaria Hungarica 46 (4), pp. 405-414 (1998)

OCCURRENCE OF SOME VIRUSES AND BACTERIA INVOLVED IN RESPIRATORY DISEASES OF RUMINANTS IN HUNGARY

M. Rusvai and L. Fodor

Department of Microbiology and Infectious Diseases, University of Veterinary Science, H-1581 Budapest, P.O. Box 22, Hungary

(Received February 5, 1998; accepted April 14, 1998)

Serological surveys, virological and bacteriological investigations were carried out on large populations of lambs and calves kept in intensive fattening units on Hungarian large-scale farms, in order to study the occurrence of viruses and bacteria involved in the respiratory disease complex. The investigations were carried out in stocks where 5-20% of the young animals succumbed to acute or chronic pneumonia every year. Serological surveys revealed that seropositivity to bovine adenovirus type 2 (BAV-2) and parainfluenza type 3 (PI-3) were the most widespread viral infections both in cattle and sheep (BAV-2: 56% and 88%, PI-3: 76% and 41%, respectively). Antibodies against infectious bovine rhinotracheitis virus (BHV-1) were also found in a high proportion (45%) of sera of 3-8 month old calves, while in 2-6 month old lambs ovine adenovirus 1 (OAV-1) was the third most frequent virus (22%). Bacteriological investigations demonstrated secondary infections caused by different Pasteurella haemolytica and Pasteurella multocida serotypes in most cases. In cattle herds P. multocida A proved to be the predominant bacterium: it was isolated in each herd examined, while P. haemolytica A1 strains were isolated in 7 and Haemophilus somnusin 2 out of 13 herds. In sheep flocks P. haemolytica was the most frequent species isolated, 10 serotypes were cultured from pneumonic sheep but serotypes A2, A1 and A8 were the dominant ones.

Key words: Cattle, sheep, respiratory diseases, adenovirus, parainfluenza-3 virus, reovirus, infectious bovine rhinotracheitis, IBR, Pasteurella multocida, Pasteurella haemolytica, Haemophilus somnus, Hungary


Acta Veterinaria Hungarica 46 (4), pp. 415-420 (1998)

DETECTION OF LAWSONIA INTRACELLULARIS IN HUNGARIAN SWINE HERDS 
BY POLYMERASE CHAIN REACTION

I. Biksi1*, I. Kacskovics2, Mira Mándoki1, Judit Iván3, I. Horváth-Papp4, G. Makay4 and F. Vetési1

1Department of Pathology and Forensic Veterinary Medicine, 2Department of Physiology and Biochemistry, University of Veterinary Science, P.O. Box 2, H-1400 Budapest, Hungary; 3Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary; 4ELANCO Animal Health, Vienna, Austria

(Received May 5, 1998; accepted May 25, 1998)

Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian swine farms and subjected to DNA extraction/purification and subsequent polymerase chain reaction (PCR) in order to detect Lawsonia intracellularis, the aetiological agent of the porcine proliferative enteropathy complex. Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from eight herds. The PCR products of collected faecal samples hybridised in Southern blot hybridisation with the DNA of the type strain L. intracellularis NCTC 12657. This is the first confirmed detection ofL. intracellularis by PCR in Hungary.

Key words: Lawsonia intracellularis, porcine proliferative enteropathy complex, PCR, Southern blot hybridisation

to whom correspondence should be addressed


Acta Veterinaria Hungarica 46 (4), pp. 421-429 (1998)

EFFECT OF METHIONINE AND ITS DERIVATIVES ON THE WEIGHT GAIN AND PROTEIN UTILISATION OF GROWING RATS

M. Hegedűs, S. Fekete, Emese Andrásofszky and I. Hullár

Department of Animal Breeding and Nutrition, University of Veterinary Science, 
H-1400 Budapest, P.O. Box 2, Hungary

(Received February 4, 1998; accepted March 16, 1998)

Methionine and its derivatives are used as feed supplements. The objective of this study was to compare the effectiveness of DL-methionine (MET), DL-methionine-hydroxy-analogue (2-hydroxy-4-methylthio-butyric acid, MHA), and DL-S-methyl-methionine-sulphonium-chloride (SMM) in rats using low- (10 g/100g, BD10), medium- (15 g/100g, BD15) and high-protein (20 g/100g, BD20) diets. In a 10-day growth assay with weaning male rats (Wistar, SPF, LATI, Gödöllő, Hungary, 6 rats/group) methionine-deficient basic diets (BD10, BD15, BD20) were supplemented with 0, 1.5 and 3.0 g/kg MET, MHA or SMM. Extracted soybean meal was used as sole protein source. A protein-free diet was used for determining net protein ratio (NPR) indices. The weight gain of rats fed test diets fortified with 1.5 and 3.0 g/kg MET, MHA or SMM was significantly higher (p < 0.05) than that obtained with the unfortified diets. The NPR index of the diets was increased by MET, MHA or SMM supplementation, which increase was significant (p < 0.05) at 3.0 g/kg fortification level. However, there were no significant differences between the efficiencies of methionine derivatives on the same dietary protein levels. The weight gains of the rats were higher and the NPR indices of the diets were lower at a higher dietary protein level.

Key words: Methionine, derivatives, methionine-hydroxy-analogue, S-methyl-methionine, protein utilisation, rats


Acta Veterinaria Hungarica 46 (4), pp. 431-436 (1998)

DETECTION OF ANTIBODIES TO NEOSPORA CANINUM IN BOVINE POSTABORTION BLOOD SAMPLES 
FROM HUNGARY

S. Hornok1, K. Näslund2, I. Hajtós4, J. Tanyi5, L. Tekes6, I. Varga1, A. Uggla2 
and C. Björkman3

1Department of Parasitology and Zoology, University of Veterinary Science, H-1078 Budapest, István u. 2, Hungary; 2Department of Parasitology, National Veterinary 
Institute and 3Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, Uppsala, Sweden; 4County Veterinary Station, 
Borsod-Abaúj-Zemplén, Miskolc, Hungary; 5Veterinary Institute, Debrecen, Hungary; 
6Central Veterinary Institute, Budapest, Hungary

(Received February 18, 1998; accepted March 30, 1998)

In order to obtain data on the occurrence of the protozoan parasite Neospora caninum as a potential factor in the aetiology of reproduction problems in cattle, 97 postabortion sera were examined by ISCOM ELISA and IFAT for the presence of antibodies to N. caninum. The results showed 10% and 9% seropositivity by the ELISA and IFAT, respectively. In 2 of the 10 seropositive cases no other fetopathogenic agents (such as Chlamydia sp., Leptospira spp. or bovine viral diarrhoea virus) were detected. These data confirm the presence of N. caninum in cattle in Hungary.

Key words: Neospora caninum, abortion, cattle, ISCOM ELISA, IFAT


Acta Veterinaria Hungarica 46 (4), pp. 437-450 (1998)

STUDIES ON THE OCCURRENCE OF ACTINOSPOREAN STAGES OF MYXOSPOREANS IN LAKE BALATON, HUNGARY, WITH THE DESCRIPTION OF TRIACTINOMYXON, RAABEIA AND AURANTIACTINOMYXON TYPES

Amina El-Mansy*, Cs. Székely** and K. Molnár

Veterinary Medical Research Institute, Hungarian Academy of Sciences,
H–1581 Budapest, P.O. Box 18, Hungary

(Received February 10, 1998; accepted March 25, 1998)

In the framework of a one-year survey, the actinosporean infection of the oligochaete fauna living in the mud and on the vegetation of Lake Balaton, Hungary was studied. A total of 10 types of actinospores were isolated from the oligochaetes Tubifex tubifex and Limnodrilus hoffmeisteri collected during the survey, which could be classified into the triactinomyxon, raabeia and aurantiactinomyxon groups. The drawings depicting the individual actinospore types are presented on a plate and their characteristic dimensions summarised in tables. The prevalence and seasonality of actinosporean infections observed in the two oligochaete species are illustrated graphically. Infection by actinospores showed pronounced seasonality. In the spring and summer the prevalence of triactinomyxon infection in T. tubifex exceeded 30 and 40%, respectively, while in the autumn it dropped to 6%. In T. tubifex raabeia infection of 3% prevalence was detected only in the summer period. In L. hoffmeisteri the peak of aurantiactinomyxon, raabeia and triactinomyxon infection occurred in the summer with a prevalence of 21, 10 and 8%, respectively. Actinosporean infection in the individual Oligochaeta species showed higher prevalence values than had been reported in the literature, which fact may be explained by the novelty of the examination technique used. Four types of actinospores found in Lake Balaton are identical with the actinospores already described. It cannot be determined with absolute certainty which stages of myxosporeans parasitic in Lake Balaton fishes are identical with the 10 actinospores described.

Key words: Actinospore, myxosporeans, Myxozoa, survey, Lake Balaton, triactinomyxon, raabeia, aurantiactinomyxon

* On leave from the National Institute of Oceanography and Fisheries, Cairo, Egypt; Student of the doctoral program in Zootaxonomy, Synbiology and Hydrobiology at the Loránd Eötvös University, Budapest

** Corresponding author. Fax: +36-1-2521069


Acta Veterinaria Hungarica 46 (4), pp. 451-463 (1998)

OCCURRENCE OF SKRJABILLANID NEMATODES IN FISHES OF HUNGARY AND IN THE INTERMEDIATE HOST, ARGULUS FOLIACEUS L.

K. Molnár and Cs. Székely

Veterinary Medical Research Institute, Hungarian Academy of Sciences, 
H-1581 Budapest, P.O. Box 18, Hungary

(Received February 26, 1998; accepted April 13, 1998)

Histozoic and coelozoic skrjabillanid-type nematodes belonging to the genera Skrjabillanus, Molnaria, Sinoichthyonema, Esocinema, Daniconema and Lucionema are recorded from the subcutaneous tissues, fins, swimbladder and abdominal cavity of different fish species living in natural waters and fish farms of Hungary. In addition to the nine taxonomically identified parasite species, one Skrjabillanus sp., two Molnaria spp. and three Esocinema spp. were identified to the genus level only. The histozoic larval stages of a Molnaria, Daniconema and Lucionema species each, living in a site different from that of the imagoes, were also detected. The presence of closely not identified first- to third-stage skrjabillanid larvae was demonstrated in 26.3% of the parasitic carp lice (Argulus foliaceus L.) collected from the eight fish species.

Key words: Nematoda, Skrjabillanidae, Lucionematidae, Daniconematidae, larval stages, fish hosts, Argulus foliaceus, intermediate host


Acta Veterinaria Hungarica 46 (4), pp. 465-471 (1998)

PHILOMETRA KARUNENSIS SP. N. (NEMATODA: PHILOMETRIDAE) FROM BARBUS SHARPEYI (PISCES) IN FRESHWATERS OF SOUTHWEST IRAN

Jamileh Pazooki1 and K. Molnár2*

1Iranian Fisheries Research and Training Organisation, Department of Fish Diseases, P.O. Box 14155-6116 Tehran, Iran; 
2Veterinary Medical Research Institute, Hungarian Academy of Sciences, H-1581 Budapest, P.O. Box 18, Hungary

(Received March 2, 1998; accepted April 20, 1998)

During a study on some Barbus species (Pisces: Cyprinidae) of the Mesopotamian fauna region (the south-western part of Iran) a new nematode species, Philometra karunensis sp. n., was found in the swimbladder (under the serosa cover) and abdominal cavity of Barbus sharpeyi (Günther). Males distinctly differ from the known species in the length of the spicules and gubernaculum, while subgravid females are very similar to those of Philometra spp. known from the abdominal cavity of cyprinid fishes.

Key words: Nematoda, PhilometraBarbus sharpeyi, Iran

*Corresponding author


Acta Veterinaria Hungarica 46 (4), pp. 473-485 (1998)

3,3-,5-TRIIODOTHYRONINE (T3) UPTAKE AND EXPRESSION OF THYROID HORMONE RECEPTORS DURING THE ADAPTATION TO HYPOTHYROIDISM OF THE BRAIN OF CHICKEN

B. Gereben1, A. Kollár1, T. Bartha1, N. Buys2, E. Decuypere2 and P. Rudas1*

1Department of Physiology and Biochemistry, University of Veterinary Science,
H-1400 Budapest, P.O. Box 2, Hungary; 2Laboratory for Physiology and Immunology
of Domestic Animals, Faculty of Agronomy, Catholic University of Leuven, Belgium

(Received February 4, 1998; accepted March 16, 1998)

Thyroid hormone action in the brain is strictly regulated, since these hormones play a crucial role in the development and physiological functioning of the central nervous system. Hormone kinetics and molecular events at the nuclear receptor level during the adaptation of the brain of chicken to hypothyroidism were simultaneously investigated. Data obtained by Oldendorff’s „single-pass” technique showed a significantly higher labelled 3,3-,5-triiodothyronine (125I-T3) uptake into the brain of surgically thyroidectomized (TX) 2-week-old broilers after 1 week of the surgery in comparison to sham-operated (SH) and T3 supplemented (TX + T3) controls in the 10th second after the bolus injection. Telencephalons showed the highest, while cerebellum the lowest uptake intensity in all groups. In a similar arrangement of experiments the expression of the TRa - and TRb nuclear thyroid receptors in the telencephalon of TX and control chickens was investigated by a semiquantitative RT-PCR study in the following way: cDNAs were standardised using a quantitative RT-PCR-based approach for b -actin, then amplified for thyroid receptors. The level of both the TRa and TRb coding mRNA was elevated in hypothyroidism. In conclusion, the presented hormone kinetics and TR expression data provide further details of the cellular and molecular events occurring during the adaptation to hypothyroidism of the brain of chicken.

Key words: Brain, chicken, thyroid hormones, thyroid receptors, kinetics of thyroid hormone, uptake

*Corresponding author


Acta Veterinaria Hungarica 46 (4), pp. 487-492 (1998)

ENDOSCOPIC METHODS IN SWINE REPRODUCTIVE RESEARCH: A REVIEW

J. Rátky1, K.-P. Brüssow2 and L. Solti3

1Research Institute for Animal Breeding and Nutrition, H-2053 Herceghalom, Hungary; 
2Research Institute for the Biology of Farm Animals, Dummerstorf, Germany; 
3Univesity of Veterinary Science, Budapest, Hungary

(Received January 30, 1998; accepted March 2, 1998)

One of the major objectives of applying clinical methods is to get a more profound knowledge of the state of an organism. With the expansion of knowledge in physiology as well as the perfection of techniques the different applications have also improved. Over the past decade it has become obvious that in swine reproductive research such techniques are needed which do not influence the physiological processes and guarantee animal welfare. The minimal invasive and non-invasive methods (transabdominal endoscopy and ultrasonography) developed in human medical practice can be successfully adapted to the requirements of swine reproductive research. The present paper reports on the experience gained with abdominal endoscopy in swine so far.

Key words: Endoscopy, reproduction, diagnostic, surgery, swine


Acta Veterinaria Hungarica 46 (4), pp. 493-500 (1998)

COMPARISON OF DIFFERENT TREATMENTS FOR PARTHENOGENETIC ACTIVATION 
OF BOVINE OOCYTES MATURED IN VITRO

Sz. Bodó1, A. Dinnyés2, B. Baranyai1, L. Solti3 and J. Dohy2

1Agricultural Biotechnology Centre, H-2001 Gödöllő, Szent-Györgyi Albert u. 4, Hungary;
2University of Agricultural Sciences, Faculty of Agriculture, Gödöllő, Hungary; 
3University of Veterinary Science, Budapest, Hungary

(Received November 13, 1997; accepted March 27, 1998)

In vitro matured bovine oocytes were treated by three parthenogenetic activation methods. Basic treatment with Ca-ionophore and cytochalasin D was combined with cycloheximide (Group 2), electric impulse (Group 3), and cycloheximide and electric pulse (Group 1) treatments, respectively. Survival and the in vitrodevelopment of parthenogenetic embryos to hatched blastocyst stage were compared. Rates of the first cleavage and morula development significantly differed among the treatment groups. Further development rates of the treated embryos up to blastocyst stage did not differ. The best results were obtained by the combination of cycloheximide and electric treatments (Group 1; 14% blastocyst, 7% hatched blastocyst). The results suggest that the combined treatment for oocyte activation is the most efficient and should be applied for nuclear cloning procedures.

Keywords: Parthenogenesis, oocyte activation, bovine, in vitro produced embryos


Acta Veterinaria Hungarica 46 (4), pp. 501-513 (1998)

PROGESTERONE DETERMINATION IN EQUINE PLASMA USING DIFFERENT IMMUNOASSAYS

P. Nagy1, L. Solti1, Margit Kulcsár1, J. Reiczigel2, Gy. Huszenicza1
Katalin Abaváry1 and Anna Wölfling1

1Department of Obstetrics and Reproduction and 2Department of Biomathematics, 
University of Veterinary Science, H-1078 Budapest, István u. 2, Hungary

(Received January 6, 1998; accepted March 31, 1998)

Several assay systems [3H radioimmunoassay (RIA) with and without extraction; microplate enzyme-linked immunoassay (ELISA); qualitative ELISA (tube test)] were used to measure plasma progesterone concentration in mare plasma. The direct RIA showed a close correlation (R = 0.94) with the extraction RIA. The direct RIA and the microplate ELISA were compared in two different studies. In the first study 1155 samples of postpartum mares were used for progesterone determination with both assays. The ELISA resulted in more elevated values both in oestrus and dioestrus (0.19 ą 0.3 and 2.44 ą 3.62 nmol/l for oestrus, n = 436, and 8.94 ą 4.29 and 27.88 ą 18.34 nmol/l for dioestrus, n = 719, for the RIA and ELISA, respectively, R = 0.71). The evaluation of individual progesterone profiles has revealed that the microplate ELISA detects the time of ovulation at the same time as it is determined by the RIA and clinical examination. The sensitivity and specificity were calculated for different progesterone threshold values. In the second study including 7 non-pregnant, cycling mares the progesterone concentration of 240 samples was determined by both assays. Basal values (Day 0) obtained with the ELISA were higher (1.57 nmol/l) than those of the RIA (0.2 nmol/l). Both curves reached the same maximum concentration (12.11 and 12.45 nmol/l) 5 days after ovulation. The correlation between the RIA and ELISA values was high (R = 0.90). The tube test was compared to the microplate ELISA as reference using 576 plasma samples of 34 non-pregnant, non-cycling mares included in an ovulation induction study. Of these samples 118 had higher and 458 had lower values than 3.18 nmol/l. In most cases the tube test was in complete agreement with the microplate ELISA. The sensitivity, specificity, + predictive and predictive values for the tube test were 79.7%, 95.4%, 81.7% and 94.8%, respectively.

Keywords: Mare, progesterone, RIA, ELISA, microplate, mare-side test


BOOK REVIEWS

Uli Braun (ed.): Atlas und Lehrbuch der Ultraschalldiagnostik beim Rind. With the contribution of Mark FLÜCKIGER, Wolfgang KÄHN, Johann KOFLER, Christoph J. LISCHER, Peter RÜSCH, Adrian STEINER, Hans STOCKER. Parey Buchverlag, Berlin, 1997. 289 pages, 300 figures, and 75 tables. Price: DM 248. ISBN: 3-8263-3092-7.

Josef ASSHEUER and Martin SAGER: MRI and CT Atlas of the Dog. Blackwell Science Ltd., Oxford, 1997. 482 pages, with over 1200 illustrations. Price: DM 298. ISBN 3-89412-164-5.