Acta Veterinaria Hungarica 49 (4) (2001)
CONTENTS AND ABSTRACTS
Clinical veterinary medicine
Frequencies of feline blood types in Hungary
. Nóra Bagdi, Melinda Magdus, E. Leidinger, Judith Leidinger and K. Vörös 369Food hygiene
Testing of a Chemiluminescence Enzyme Immunoassay for selective detection of E. coli O157 from ground beef samples
. Hajnalka Domján Kovács and Klára Rásky 377Nutrition
Alteration of body composition in rats: Effect of organic chromium and L-carnitine
. S. Fekete, I. Szakáll, Emma Kósa, Emese Andrásofszky, Kinga Fodor, A. Hidas and J. Tőzsér 385Influence of feeding intensity on corporeal development, body composition and sexual maturity in female rabbits
. Kinga Fodor, S. Gy. Fekete, L. Zöldág, A. Bersényi, A. Gáspárdy, Emese Andrásofszky, Margit Kulcsár and F. Eszes 399Physiology
Erythrocyte osmotic fragility test as the measure of defence against free radicals in rabbits of different age
. Ewa Brzezińska-Ślebodzińska 413A simple way to reduce heat stress in laying hens as judged by egg laying, body weight gain and biochemical parameters
. K. Şahin and O. Küçük 421Reproduction
Studies on factors affecting superovulation and embryo transfer in Hungarian Merino ewes
. S. Cseh and L. Solti 431Ovarian stimulation and ultrasound-guided oocyte retrieval in baboon (Papio cynocephalus anubis) during pituitary suppression with a gnrh agonist
. S. Cseh, J. Corselli, P. Chan and L. Bailey 443Surgery
Endoscopy and autopsy follow-up of biodegradable oesophageal anastomoses in dogs. T. Kovács, T. Németh, Zs. Orosz and I. Köves
451Toxicology
Decreasing half-life of dieldrin in egg yolk following a single oral administration of aldrin to laying hens
. N. Furusawa 465Virology
EHV-1 gene63 is not essential for in vivo replication in horses and mice, nor does it affect reactivation in the horse: Short communication
. J. Iqbal, A. S. Purewal and N. Edington 473Book reviews 479
Acta Veterinaria Hungarica 49 (4), pp. 369–375 (2001)
FREQUENCIES OF FELINE BLOOD TYPES IN HUNGARY
Nóra Bagdi1*, Melinda Magdus2, E. Leidinger3, Judith Leidinger3 and K. Vörös1
1Department and Clinic of Internal Medicine, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary; 2Private practitioner, Budapest, Hungary; 3Veterinary Laboratory Invitro, Vienna, Austria
(Received March 9, 2001; accepted September 5, 2001)
Feline blood group determination is done as a routine diagnostic method in numerous countries. Blood transfusion reactions and feline neonatal isoerythrolysis (FNI) can be avoided with the identification of different feline blood groups. The present study is the first investigation in Hungary during which 100 cats have been examined from all over the country. These cats were out of six breeds: European domestic shorthair, Persian mix, Persian, Abyssinian, Siamese and British shorthair. In the Hungarian feline population European domestic shorthair are most common but other breeds also occur. European domestic shorthair, Persian mix, Abyssinian, Siamese and British shorthair individuals all belonged to blood type A (100%). Blood type B was found very rarely and only in Persian cats. One-third of the Persian cats were categorised into blood type B, whilst type AB was not found during the study.
Keywords: Feline, blood types, transfusion, neonatal isoerythrolysis
*
Corresponding author; Dr. Nóra Bagdi, H-1076 Budapest, Thököly út 4. II. 26, Hungary; E-mail: bagdiparti@freemail.hu; Fax: +36 (1) 351-5276Acta Veterinaria Hungarica 49 (4), pp. 377–383 (2001)
TESTING OF A CHEMILUMINESCENCE ENZYME IMMUNOASSAY FOR SELECTIVE DETECTION
OF E. COLI O157 FROM GROUND BEEF SAMPLESHajnalka Domján Kovács1* and Klára Rásky2
1National Food Investigation Institute, H-1465 Budapest 94, P.O. Box 1740, Hungary;
2Diagnosticum Ltd, H-1047 Budapest, Attila u. 126, Hungary(Received September 11, 2001; accepted September 28, 2001)
The aim of this study was to evaluate a Chemiluminescence Enzyme Immunoassay (CLIA) developed for the detection of E. coli O157:H7, using different E. coli O157 serotypes. The sensitivity and specificity of the kit were determined from the tenfold dilutions of the 24-hour broth cultures of the test strains. According to the results obtained in this trial, the sensitivity of the kit is 103–104 cells ml–1, and it is specific for E. coli O157. Twenty-five g ground raw beef samples were prepared and inoculated with E. coli O157:H7 at different CFU g–1. The samples were incubated in 225 ml of modified E. coli broth with novobiocin (mEC + n) at 42 °C for 4 h and the immunoassays were performed following the instructions of the manufacturer. According to the results obtained by the CLIA test 101–102 E. coli O157 g–1 can be detected from the sample. So this kit seems to be suitable for screening the samples before selective cultivation of E. coli O157:H7.
Key words: E. coli O157:H7, Chemiluminescence Enzyme Immunoassay, screening, ground beef
*
Corresponding author: Dr. Kovácsné Dr. Domján Hajnalka, H-1118 Budapest, Bakator u. 3, Hungary; E-mail: oai@oai.hu; Fax: +36 (1) 216-1574Acta Veterinaria Hungarica 49 (4), pp. 385–398 (2001)
ALTERATION OF BODY COMPOSITION IN RATS:
EFFECT OF ORGANIC CHROMIUM AND L-CARNITINES. Fekete1*, I. Szakáll1, Emma Kósa1, Emese Andrásofszky1, Kinga Fodor1,
J. Tőzsér3
A. Hidas2 and1
Szent István University, Faculty of Veterinary Science Budapest, H-1400 Budapest,
P.O. Box 2, Hungary; 2Institute for Small Animal Research, Gödöllő, Hungary;
3Szent István University, Faculty of Agricultural Science, Gödöllő, Hungary(Received July 31, 2000; accepted May 17, 2001)
A growth trial was carried out to test the effect of organic, trivalent chromium and L-carnitine on the body composition of growing rats. At the same time, an evaluation of different measurement methods (weight of epididymal fat pad, adipocyte morphometry, total body electrical conductivity) was performed. Outbred Wistar rats of 30 days of age were fed diets of different (0, 10 and 20%) protein level. The diets were supplemented with 4 mg/kg Cr as chromium nicotinate, and 100 mg/kg L-carnitine. The experimental feeding lasted 15 days, after a 5-day-long adjustment period. It was found that Cr addition increased feed intake. Both treatments caused changes in body composition, increasing fat and protein deposition. Organic chromium had no effect at either protein level, while L-carnitine improved the protein retention only at an optimum (20%) protein supply. No statistically significant correlation was found between total body electrical conductivity (TOBEC) and body composition, which could be attributed to the great individual differences. A close correlation was found among total body fat percentage, weight of epididymal fat pad and the adipocyte surface. The data suggest that there is an interaction between dietary protein supply and the effect of repartitioning agents.
Key words: Trivalent chromium, L-carnitine, body composition, protein efficiency ratio (PER), net protein ratio (NPR), rat, dietary protein level, adipocyte morphometry, epididymal fat pad, total body electrical conductivity (TOBEC), nutriceuticals
*
Corresponding author; E-mail: safekete@univet.hu; Fax: +36 (1) 478-4128Acta Veterinaria Hungarica 49 (4), pp. 399–411 (2001)
INFLUENCE OF FEEDING INTENSITY
ON CORPOREAL DEVELOPMENT, BODY COMPOSITION
AND SEXUAL MATURITY IN FEMALE RABBITSKinga Fodor, S. Gy. Fekete*, L. Zöldág, A. Bersényi, A. Gáspárdy,
Emese Andrásofszky, Margit Kulcsár and F. EszesInstitute of Animal Breeding, Nutrition and Laboratory Animal Science,
Faculty of Veterinary Science Budapest, Szent István University,
H-1400 Budapest, P.O. Box 2, Hungary(Received November 8, 2000; accepted September 5, 2001)
Twenty-six 6-week old female New Zealand White rabbits were divided into two groups: ad libitum (AL) and 70% restricted (RS) feeding. At the beginning of the experiment the liveweights were practically the same: 0.99 ± 0.08 vs. 1.01 ± 0.08 kg in group AL and RS, respectively. At 18 weeks of age the body weight of Group RS rabbits was 84.7% (3.14 ± 0.24 kg) of the group AL (3.71 ± 0.31 kg). The apparent digestibility of crude protein was significantly (p < 0.001) higher in Group RS than in Group AL (76.5 ± 1.4 vs. 73.0 ± 2.7%). The daily water consumption was significantly (p < 0.05) higher (3.5 ml/g DM intake) in Group RS as compared to Group AL (1.9 ml/g DM). Since the average body weight in Group RS at 18 weeks was the same as that of Group AL at 14 weeks of age (3.14 kg), the comparison of the live body measures and indices was based on these ages. Animals fed ad libitum or restricted show no differences at the defined age in most live body measurements except in heart girth and rump width, which were significantly (p < 0.05) shorter in Group AL than in Group RS (29.3 ± 0.8, 5.7 ± 0.5 and 30.7 ± 1.0, 6.2 ± 0.3 cm, respectively). Body capacity was significantly (p < 0.05) smaller in Group AL, as the head capacity-body capacity proportion was significantly (p < 0.05) higher in Group AL than in Group RS (1653.1 ± 134.5, 22.0 ± 2.5 and 1913.7 ± 168.7, 17.7 ± 1.9, respectively). Due to restricted feeding the growth of the head proved to be less intensive than that of the body at the same body weight. The body in these does tended to be wider. Since the head in comparison to length or capacity of the body was smaller in does fed 70% of ad libitum, it could be concluded that the development of body parts of restricted-fed does was unequal (allometric growth). The effect of feed restriction reflected in lower dry matter and fat, and a higher ash and protein content both in total body and in dry matter composition of rabbits at 18 weeks of age. Restricted feeding delayed sexual maturity (69 vs. 92% of rabbits) with later starting ovarian activity, weaker ovarian responsiveness, and a smaller number of tertiary follicles on the ovarian surface.
Key words: Restricted feeding, growing female rabbit, body measures, chemical composition, sexual maturity
*
Corresponding author; E-mail: safekete@univet.hu; Fax: +36 (1) 478-4128Acta Veterinaria Hungarica 49 (4), pp. 413–419 (2001)
ERYTHROCYTE OSMOTIC FRAGILITY TEST
Brzezińska-Ślebodzińska*
AS THE MEASURE OF DEFENCE AGAINST FREE RADICALS
IN RABBITS OF DIFFERENT AGEDepartment of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of the Polish Academy
of Sciences in Olsztyn, ul. Grunwaldzka 250, 60-166 Poznań, Poland(Received May 7, 2001; accepted September 5, 2001)
Peroxidation of the unsaturated bonds of membrane lipids increases fragility and cellular lysis of red blood cells. Erythrocyte susceptibility to the free radicals (peroxyl radicals) generated in vitro by 2,2’-azo-bis(2-amidinopropane) hydrochloride (AAPH) was evaluated and expressed as 50% maximal haemolysis time (HT50) in 3 groups of rabbits of different age. Erythrocytes of 1.5-month-old rabbits were more sensitive to free radicals than those of 3.5- and 6-month-old ones. In the three groups, significant negative correlation (r = –0.8 to –0.98) between the lipid peroxidation rate (thiobarbituric acid reactive substances; TBARS concentration) in blood plasma and the erythrocyte resistance to free radicals was found. This result suggests that the plasma antioxidant defence system is interrelated with that of the red blood cells and that the erythrocytes can be a good model for studies of oxidative stress. The simple haemolysis test reflecting the free radical defence can be useful for evaluating the antioxidant properties of various compounds.
Key words: Erythrocyte, free radicals, oxidative stress, lipid peroxidation
*
E-mail: endocrin@rose.man.poznan.pl; Fax: +48 (1033) 61-868-53-28Acta Veterinaria Hungarica 49 (4), pp. 421–430 (2001)
A SIMPLE WAY TO REDUCE HEAT STRESS IN LAYING HENS AS JUDGED BY EGG LAYING, BODY WEIGHT GAIN AND BIOCHEMICAL PARAMETERS
In* and O. KüçükDepartment of Animal Nutrition, Veterinary Faculty of Firat University, 23119 Elazig, Turkey
(Received June 20, 2001; accepted September 5, 2001)
This study was designed to test the effects of feed withdrawal and darkening on the performance, triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), adrenocorticotropic hormone (ACTH), and some blood serum metabolite and mineral concentrations of laying hens reared at high ambient temperatures ranging from 25 to 35 °C. Ninety, 16-week-old hens (Ross Brown) were divided into 3 groups, 30 hens each. The first group was used as control. Hens in the second group (feed withdrawal) were subjected to feed removal from 14:00 to 18:00, and hens in the third group (darkening) were subjected to light restriction from 14:00 to 18:00 using black curtains. Liveweight, feed intake, and egg production were higher (P < 0.01) in the feed withdrawal and darkening groups, particularly in the darkening group, than in the control. Water intake was higher in the control group compared with the feed withdrawal and darkening groups (P < 0.01). T3, T4, and TSH concentrations in the serum were higher (P < 0.01), whereas ACTH serum concentration was lower (P < 0.01) in the feed withdrawal and darkening groups compared with the control. The haematocrit was higher in the feed withdrawal and darkening groups compared with the control (P < 0.01). Darkening and feed withdrawal treatments increased serum glucose, urea-N, uric acid, albumin, triglyceride, cholesterol, Ca, P, Na, and K concentrations, also the activities of amylase and alkaline phosphatase, but did not influence the activities of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). The present study found that feed withdrawal and darkening, particularly darkening, at high temperatures during the summer months offer a good management practice to reduce heat stress related depression in feed intake and egg production in laying hens.
Key words: Laying hen, heat stress, darkening, feed withdrawal, performance
*
Corresponding author; Dr. Kazim Şahin, Veteriner Kontrol ve Araştirma Enstitüsü, 23119 Elazig, Turkey; E-mail: ksahin@firat.edu.tr; Fax: +90 (424) 233-8720Acta Veterinaria Hungarica 49 (4), pp. 431–441 (2001)
STUDIES ON FACTORS AFFECTING SUPEROVULATION
AND EMBRYO TRANSFER IN HUNGARIAN MERINO EWESS. Cseh* and L. Solti
Department and Clinic of Obstetrics and Animal Reproduction, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary
(Received April 10, 2001; accepted September 5, 2001)
The objectives of this study were (a) to assess the ovulatory response and embryo production of Hungarian Merino ewes after superovulation, (b) to investigate the factors influencing the efficiency of embryo transfer (ET) in Hungarian Merino ewes, (c) to compare the results of two ovarian stimulation protocols (PMSG and PMSG+FSH treatment) in Hungarian Merino ewes, and (d) to study how superovulation, laparoscopic insemination and surgical embryo retrieval (ER) affect the subsequent reproduction of Hungarian Merino donor females after an ET programme. There was no significant difference between the ovarian stimulation protocols in the ratio of donor ewes responding to superovulation nor in the average number of corpora lutea. However, the number of transferable embryos recovered per donor ewe was higher in the PMSG+FSH group. The proportion of transferable embryos, unfertilised oocytes and degenerated embryos did not differ between the treatment protocols. The total pregnancy rate was 53.4% (179/335). Neither the developmental stage of the embryo nor the number of transferred embryos affect the implantation of embryos. However, the increased number of transferred embryos positively influenced the pregnancy rate. No difference was found in the pregnancy rate between synchronised and non-synchronised groups of recipients. Thirty-six out of 45 donor ewes (80%) became pregnant within one year after the ET programme, indicating that ovarian stimulation and surgical ER did not affect adversely their reproduction.
Key words: Embryo transfer, superovulation, embryo developmental stages, oestrus synchronisation
*
Corresponding author; E-mail: scseh@univet.hu; Fax: +36 (26) 360-499Acta Veterinaria Hungarica 49 (4), pp. 443–450 (2001)
OVARIAN STIMULATION AND ULTRASOUND-GUIDED
OOCYTE RETRIEVAL IN BABOON (PAPIO CYNOCEPHALUS ANUBIS) DURING PITUITARY SUPPRESSION WITH A GNRH AGONIST*S. Cseh1**, J. Corselli1, P. Chan1 and L. Bailey2
1Department of Obstetrics and Gynaecology, Faculty of Veterinary Science Budapest, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary; 2Department
of Surgery, School of Medicine, Loma Linda University, Loma Linda, CA, USA, 92350(Received April 18, 2001; accepted September 5, 2001)
The objective of this study was to investigate whether baboon females respond to an ovarian stimulation protocol incorporating pituitary suppression with a GnRH agonist (GnRHa) and either highly purified human FSH (hphFSH) or recombinant human FSH (rhFSH) with follicular development and oocyte maturation. A modified human ovulation induction protocol was applied to 5 adult female baboons with a history of regular menstrual cycles (33–34 days). A long-acting GnRHa implant containing goserelin acetate was placed subcutaneously (s.c.) on Days 22–24 of their menstrual cycle. Concentrations of serum oestradiol (E2), progesterone (P4) and human FSH were obtained by ELISA. Menses occurred ~ 10 days after GnRHa implantation. Daily hphFSH or rhFSH (75 IU i.m.) treatments were started ~ 10 days following menses. When the majority of follicles were ? 5 mm in diameter and the E2 levels had reached a maximum, hCG (2000 IU i.m.) was administered to induce final maturation of oocytes and ovulation. Thirty to 34 h after hCG administration, transabdominal follicular aspiration was performed using a variable frequency transvaginal transducer with ultrasound. A total of 71 oocytes were collected from 4 animals (average: 17). The meiotic maturity of oocytes was evaluated 3 h after retrieval. Ninety-one percent of oocytes were in metaphase 2 and of grades I and II which are appropriate for in vitro insemination.
Key words: Ovarian stimulation, GnRH agonist, ultrasound-guided oocyte retrieval, baboon
**
Corresponding author; Dr. Sándor Cseh, H-1400 Budapest, Pf. 2, Hungary; E-mail: scseh@univet.hu; Fax: +36 (26) 360-499Acta Veterinaria Hungarica 49 (4), pp. 451–463 (2001)
ENDOSCOPY AND AUTOPSY FOLLOW-UP
OF BIODEGRADABLE OESOPHAGEAL ANASTOMOSES IN DOGST. Kovács1*, T. Németh2, Zs. Orosz3 and I. Köves1
1Department of General and Thoracic Surgery, National Institute of Oncology,
H-1122 Budapest, Ráth Gy. u. 7–9, Hungary; 2Department and Clinic of Surgery and Ophthalmology, ‘Szent István’ University, Faculty of Veterinary Science, Budapest, Hungary; 3Department of Pathology, National Institute of Oncology, Budapest, Hungary(Received May 25, 2001; accepted October 5, 2001)
The Biofragmentable Anastomosis Ring (BAR) is a mechanical device composed of absorbable material and creates an inverting, atraumatic compressive anastomosis with no foreign material at the anastomotic site after healing. The aim of the present experimental study was to assess the safety of oesophagoscopy in early days after oesophageal anastomoses performed with the BAR and to follow-up the healing of BAR anastomoses by in vivo endoscopy and autopsy examination. Thirty mongrel dogs divided into subgroups according to the time-points of endoscopy and autopsy (4th, 7th, 14th, 28th day) were used. There was no significant difference in the healing of anastomoses performed under or above the tracheal bifurcation. Pleural adhesions helped to cover and seal small subclinical leaks. The mortality was 13.3% (4 dogs) and the overall leakage rate 14.3%. We looked for bleeding, haematoma, erosion, ulceration and granulation tissue in the anastomosis. Due to the high mechanical strength of these anastomoses, oesophagoscopy was a safe, easy and feasible method for follow-up BAR intrathoracic anastomoses, with no significant difference between the number of lesions found with endoscopy as compared to the autopsy data. The overall sensitivity of oesophagoscopy to discover mucosal lesions was 73.1%. Endoscopy had no complications, therefore it is a useful method of follow-up and may help predict the normal or compromised healing of oesophageal anastomoses.
Key words: Endoscopy, autopsy follow-up, biofragmentable oesophageal anastomosis, dog
*
Corresponding author; E-mail:kovacsti@oncol.hu; Fax: +36 (1) 224-8620Acta Veterinaria Hungarica 49 (4), pp. 465–472 (2001)
DECREASING HALF-LIFE OF DIELDRIN IN EGG YOLK
FOLLOWING A SINGLE ORAL ADMINISTRATION
OF ALDRIN TO LAYING HENSFaculty of Human Life Science, Osaka City University, Osaka 558-8585, Japan
(Received May 2, 2001; accepted September 5, 2001)
µg/g) of AD or its epoxide (= dieldrin, DD) in the yolk of eggs laid for 21 days after AD treatment were determined by normal-phase high-performance liquid chromatography. The limits of determination were 0.02 µg/g for AD and 0.03 µg/g for DD, respectively. After AD treatment, although the low levels of AD (mean 0.02–0.03 µg/g) were observed only during a three-day period (from 4th to 6th days), DD (mean 0.15 µg/g) was found already on the 2nd day, indicating that the epoxidation of AD to DD in the hen’s body is rapid. The highest level of DD (mean 0.40 µg/g) was detected on the 6th day, and then DD levels decreased slowly and were detected up to the 21st day. In this decreasing phase, the half-life of DD in the yolk was estimated to be 25.6 days with a 95% confidence interval from 22.7 to 29.4 days.Laying hens were treated orally with a single dose of aldrin (AD) 1 mg/kg body weight. Concentrations (
Keywords: Aldrin, dieldrin, epoxidation, laying hens, egg yolk
*
E-mail: furusawa@life.osaka-cu.ac.jp; Fax: +81-6-6605-2864Acta Veterinaria Hungarica 49 (4), pp. 473–478 (2001)
EHV-1 GENE63 IS NOT ESSENTIAL FOR IN VIVO
REPLICATION IN HORSES AND MICE,
NOR DOES IT AFFECT REACTIVATION IN THE HORSE:
SHORT COMMUNICATIONJ. Iqbal*, A. S. Purewal and N. Edington
Department of Veterinary Basic Sciences and Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
(Received May 2, 2001; accepted September 5, 2001)
The aim of this study was to investigate the role of immediate early gene (gene63) in the pathogenesis of equine herpesvirus 1 (EHV-1) acute and latent infections in equine and murine models. EHV-1 gene63 mutant virus (g63mut) along with EHV-1 (Ab4) was used for intracerebral and intranasal infection of 3 and 17-day-old mice. Both viruses were recovered at the same frequency from tissues after infection. Two Welsh ponies were infected via the intranasal route with each of the viruses. Acute infection was monitored by virus isolation from nasal swabs and peripheral blood leukocytes. Six weeks post infection, peripheral blood leukocytes were taken from ponies and in vitro reactivation was positive for both viruses. At autopsy, both viruses were isolated by co-cultivation from bronchial and submandibular lymph nodes. These findings indicate that the mutation of EHV-1 gene63 does not play a role in the establishment and reactivation from latency.
Key words: EHV-1, gene63, mice, horses, latency, reactivation
*
Corresponding author: J. Iqbal, VBS Department, Royal Veterinary College, Hawkshead Lane, Hatfield, Herts AL9 7TA, UK; E-mail: jiqbal@rvc.ac.uk; Fax: 01707 652090Acta Veterinaria Hungarica 49 (4), pp. 479–486 (2001)
BOOK REVIEWS
Manual of Standards for Diagnostic Tests and Vaccines. Fourth edition, 2000. Office International des Épizooties, 12, rue de Prony, 75017 Paris, France. 957 pages, 29.7 × 21 cm in plastic hardcover. Price: 120 euros.
International Aquatic Animal Health Code. Third edition, 2000. Fourth edition, 2001. Office International des Épizooties, 12, rue de Prony, 75017 Paris, France. 164 pages. Format 21 × 29.7 cm. ISBN 92-9044-541-6. Price: 40 euros. Diagnostic Manual for Aquatic Animal Diseases. Third edition, 2000. Office International des Épizooties, 12, rue de Prony, 75017 Paris, France. 270 pages. 21 × 29.7 cm. ISBN 92-9044-538-6. Price: 40 euros.
Hans G. Niemand and Peter F. Suter: Canine Clinical Practice (Praktikum der Hundeklinik). Editor: Peter S. Suter with the contribution of 27 co-workers. Ninth revised and enlarged edition, Parey Buchverlag, Berlin 2001. Hardcover, 1361 pages, 458 figures, 83 of which are coloured. Price: 298 DEM (126.80 euros). ISBN: 3-8263-3154-0.
Marc Vandevelde, André Jaggy, Johann Land (eds): Veterinary Neurology (in German). A Guide for Study and Practice. 2nd edition, 280 pages with 148 figures and 38 tables. ISBN: 3-8263-3224-5. Price: 148.00 DEM (75.67 EUR).
Eckert, J., Gemmell, M. A., Meslin, F.-X., Pawłowski, Z. S. (eds): WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Co
ncern. Office International des Épizooties (World Organisation for Animal Health), Paris and World Health Organisation, Geneva, 2001. 265 pages, with 53 figures, 55 tables, paperback, 21 × 30 cm. ISBN 92-9044-522-X.