Forthcoming!

ADENOVIRUS METHODS AND PROTOCOLS

Edited by William S. M. Wold, St. Louis University Health Sciences Center, St. Louis, MO

The prominent researchers that have contributed to this volume provide practical step-by-step adenovirus (Ad) protocols useful throughout Ad research today.

CONTENTS: Preparation and Titration of CsCL-banded Adenovirus Stocks. Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Unit. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants. Manipulation of Early Region 4. Adenovirus DNA Packaging-Construction and Analysis of Viral Mutants. Methods for Creating and Analyzing Adenovirus Vectors Which Express Proteins that Act on the Viral Genome. Construction of Mouse Adenovirus Type 1 Mutants. Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells. Measurement of TNF (Tumor Necrosis Factor) Lysis of Adenovirus-Infected Cells. Role of NK Cells in Immunity to Adenoviruses. Determination of the Transforming Activities of Adenovirus Oncogenes. Cell Survival and Apoptosis Assays for ElB 19K and Bcl-2 Family Members. In Vitro Transcription: Probing the Molecular Functions of Adenovirus Regulatory Proteins. Cell Microinjection: In vivo Analysis of the Functional Domains of Viral Regulatory Proteins. Immunoprecipitation of ElA-Containing Protein Complexes. Preparation of Splicing Competent Nuclear Extracts from Adenovirus Infected Cells. Adenovirus Entry Into Cells: A Quantitative Fluorescence Microscopy Approach. Simultaneous Detection of RNA and Proteins in Adenovirus Infected Cells by Fluorescence In Situ Hybridization and Immunostaining. Characterization of the Adenovirus Fiber Protein. Large Scale Purification and Crystallization of Adenovirus Hexon. Adenovirus Protease. Methods for Growth and Purification of Enteric Adenovirus Type 40. Transfection Complexes Generated with Adenovirus and Polyethyleneimine Condensed DNA. Phylogenetic Analysis of Adenovirus Sequences: Proof of the Necessity of Establishing a Third Genus in the Adenoviridae Family. Index.

ca 450 pp. * July 1998 * Methods in Molecular Medicine^TM

Hardcover * 0-89603-551-4 * $79.50 (T)

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